Evidence map›Paper›PMID 40071650›Full record

ArticleAdvanced biology2025

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles.

Paula De Dios Andres, Mousumi Akter, Cecilie Ryberg, Brigitte Städler, Allen P Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Flippase-Mediated Hybrid Vesicle Division.Advanced materials (Deerfield Beach, Fla.) · 2025
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Paula De Dios AndresInterdisciplinary Nanoscience Center (iNANO), Aarhus University, Aarhus, 8000, Denmark.ORCID https://orcid.org/0000-0003-3315-5319
Mousumi AkterDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan, 48109, USA.
Cecilie RybergInterdisciplinary Nanoscience Center (iNANO), Aarhus University, Aarhus, 8000, Denmark.
Brigitte StädlerInterdisciplinary Nanoscience Center (iNANO), Aarhus University, Aarhus, 8000, Denmark.ORCID https://orcid.org/0000-0002-7335-3945
Allen P LiuDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan, 48109, USA.ORCID https://orcid.org/0000-0002-0309-7018

Funding

Army Research Office 80523-BBH2020 European Research Council 818890National Science Foundation MCB 2201236
6 · The paper itself

Abstract

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5'-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Indexed as

Artificial CellsMicrotubulesPolymersGuanosine TriphosphatePhosphatidylcholinesPolymerization1,2-oleoylphosphatidylcholineGuanosine TriphosphatePhosphatidylcholinesPolymersartificial cellsgiant hybrid vesiclesmicrotubulestubulin polymerization

Identifiers

PMID40071650
PMCPMC12078856

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.