Evidence map›Paper›PMID 40070036›Full record

ArticleBiotechnology and applied biochemistry2025

Fabrication of Polypyrrole-Polystyrene Sulfonate by Amperometric Biosensor-Based Electropolymerization for Cholesterol Determination in Serum.

Alaa Anwer Ali Dada, Ertan Yildirim, Sinan Mithat Muhammed, Servet Çete

Abstract read
In one paragraph

Article in Biotechnology and applied biochemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Alaa Anwer Ali DadaInstitue of Science, Gazi University, Ankara, Türkiye.
Ertan YildirimDepartment of Chemistry, Faculty of Science, Gazi University, Ankara, Türkiye.
Sinan Mithat MuhammedTechnical Sciences Vocational School, Gazi University, Ankara, Türkiye.
Servet ÇeteDepartment of Chemistry, Faculty of Science, Gazi University, Ankara, Türkiye.ORCID https://orcid.org/0000-0002-9316-2812

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Since cholesterol triggers many diseases, many methods have been developed for its determination. In this study, an alternative system for cholesterol determination was developed by preparing amperometric biosensors. In the development of the biosensor, Pt/polypyrrole-polystyrene sulfonate film was prepared by electropolymerization of polypyrrole in polystyrene sulfonate medium using platinum surfaces. The cholesterol oxidase enzyme was immobilized on the prepared platinum/polypyrrole-polystyrene sulfonate electrode. For molecular determination in the prepared cholesterol biosensor, a series of enzymatic reactions were performed on the enzyme electrode surface at +0.40 V by utilizing the oxidation of hydrogen peroxide. The effects of environmental conditions such as temperature and pH that affect the performance of the biosensor were investigated, and the most suitable conditions for the biosensor were determined. The linear working range of the amperometric biosensor for cholesterol determination was determined. In enzyme immobilization, calculations were made for the Michaelis-Menten constants K

Indexed as

Biosensing TechniquesCholesterolElectrochemical TechniquesPolymersPolystyrenesPyrrolesCholesterol OxidaseElectrodesEnzymes, ImmobilizedHumansHydrogen-Ion ConcentrationPolymerizationCholesterolCholesterol OxidaseEnzymes, ImmobilizedPolymerspolypyrrolePolystyrenespolystyrene sulfonic acidPyrrolesbiosensorblood samplescholesterolpolypyrrolepolystyrene sulfonate

Identifiers

PMID40070036
PMCPMC12529461

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.