Evidence map›Paper›PMID 40069560›Full record

ArticleCommunications biology2025

The core microbiomes and associated metabolic potential of water kefir as revealed by pan multi-omics.

Samuel Breselge, Iwona Skibinska, Xiaofei Yin, Lorraine Brennan, Kieran Kilcawley, Paul D Cotter

Abstract read
In one paragraph

Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Samuel BreselgeTeagasc Food Research Centre, Moorepark, Cork, Ireland.ORCID http://orcid.org/0000-0003-1738-7902
Iwona SkibinskaTeagasc Food Research Centre, Moorepark, Cork, Ireland.ORCID http://orcid.org/0009-0003-0933-4138
Xiaofei YinUCD Institute of Food and Health, UCD School of Agriculture and Food Science, University College Dublin, Dublin, Ireland.
Lorraine BrennanUCD Institute of Food and Health, UCD School of Agriculture and Food Science, University College Dublin, Dublin, Ireland.
Kieran KilcawleyTeagasc Food Research Centre, Moorepark, Cork, Ireland.ORCID http://orcid.org/0000-0003-4048-8883
Paul D CotterTeagasc Food Research Centre, Moorepark, Cork, Ireland. Paul.Cotter@teagasc.ie.ORCID http://orcid.org/0000-0002-5465-9068

Funding

Department of Agriculture, Food and the Marine (DAFM) SFI/16/RC/3835EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020) 818368Enterprise Ireland TC/2018/0025Science Foundation Ireland (SFI) SFI/12/RC/2273_P2Science Foundation Ireland (SFI) USIRL-2019-1
6 · The paper itself

Abstract

Water kefir (WK) is an artisanal fermented beverage made from sugary water, optional fruits and WK grains. WK grains can be reused to start new fermentations. Here we investigate the microbial composition and function of 69 WK grains and their ferments by shotgun metagenomics. A subset of samples was subjected to metabolomic, including volatilomic, analysis. The impact of different fermentation practices on microbial composition and fermentation characteristics was analysed and it was noted that, for example, the common practice of drying water kefir grains significantly reduces microbial diversity and negatively impacts subsequent grain growth. Metagenomic analysis allowed the detection of 96 species within WK, the definition of core genera and the detection of different community states after 48 h of fermentation. A total of 485 bacterial metagenome assembled genomes were obtained and 18 putatively novel species were predicted. Metabolite and volatile analysis show associations between key species with flavour compounds. We show the complex microbial composition of WK and links between fermentation practices, microbes and the fermented product. The results can be used as a foundation for the selection of species for large scale WK production with desired flavour profiles and to guide the regulatory framework for commercial WK production.

Indexed as

BacteriaKefirMicrobiotaFermentationMetabolomicsMetagenomeMetagenomicsMultiomics

Identifiers

PMID40069560
PMCPMC11897133

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.