ArticleMethods in molecular biology (Clifton, N.J.)2025
Reference-Based Gene Expression Analysis Using Galaxy Server.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
RNA sequencing (RNA-Seq) has become the gold standard for transcriptome analysis, making gene expression quantification the core application in molecular biology research. For well-annotated genomes, the standard RNA-Seq workflow relies on a reference genome-based approach. However, RNA-Seq analysis presents different challenges, including complex computational pipelines, the need for bioinformatics skills, and significant computational requirements. The Galaxy platform addresses these challenges by providing an easy-to-use, web-based solution that integrates multiple bioinformatics tools and databases. This enables researchers without extensive bioinformatics expertise to effectively analyze RNA-Seq data. This chapter aims to describe the process and use of the tools required to perform a reference-based RNA-Seq gene expression analysis using the Galaxy public web server.
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