ArticleMethods in molecular biology (Clifton, N.J.)2025
Cell-Type-Specific Protein Metabolic Labeling and Identification Using the Methionine Subrogate ANL in Cells Expressing a Mutant Methionyl-tRNA Synthetase.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The study of protein homeostasis in vivo is crucial for our understanding of the functions of cells and organisms. However, complex organisms, such as mammals, are built from heterogeneous tissues and cell-types. These cell-types are often specialized and react in different ways to the same physiological or pathological stimulus. Therefore, a major challenge in proteomics is the identification of proteomes and their behavior in a cell-type-specific manner. In this protocol, we describe a technique to label, enrich, and identify proteins from specific cell types. This technique is based on the expression of a mutant methionyl-tRNA synthetase (MetRS*) for incorporation of a bioorthogonal analog of methionine (ANL) into proteins. ANL can be subsequently bound to an alkyne by click-chemistry, which is used as a bait for protein purification followed by mass spectrometry identification.
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