Evidence map›Paper›PMID 40067618›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Neurospheres from Ovarian Cortical Cells in Mammals: Generation In Vitro and Neurosphere Assay.

Alfredo González-Gil, Rosa Ana Picazo

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Alfredo González-GilDepartment of Physiology, School of Veterinary Medicine, Complutense University of Madrid, Madrid, Spain.ORCID 0000-0002-5802-8028
Rosa Ana PicazoDepartment of Physiology, School of Veterinary Medicine, Complutense University of Madrid, Madrid, Spain. rapicazo@ucm.es.ORCID 0000-0003-0346-5623

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Isolation of neural stem cells/neural progenitor cells (NSC/NPC) from extra-neural origin, or adult stem cells able to specify into NSC/NPC in vitro under particular cell culture conditions, offers an interesting alternative experimental model with prospective benefits in basic research and translational medicine.In this chapter, we describe a procedure of isolation and culture of ovarian cortical cells that consistently allows the spontaneous generation of a large number of neurospheres (NS), and the methods to demonstrate that their integrating NSC/NPC self-renew and are able to differentiate into neurons and glia, during the NS assay.

Indexed as

Cell Culture TechniquesNeural Stem CellsOvarySpheroids, CellularAnimalsCell DifferentiationCells, CulturedCell SeparationFemaleMiceNeuronsNeural progenitorsNeural stem cellsNeurosphere assayNeurospheresOvarian cortical cells

Identifiers

PMID40067618

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.