ArticleMethods in molecular biology (Clifton, N.J.)2025
Flow Cytometry-Based Protocols for the Mouse Subependymal Neurogenic Niche Phenotyping.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- A validated FACS-based protocol for the isolation of neuronal and non-neuronal cells from frozen adult brain, suitable for integrated gene and protein analysis.Pharmacological reports : PR · 2026Article
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6 authors.
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Abstract
The cellular compartment of the adult rodent subependymal zone (SEZ)-neurogenic niche is the most active regenerative area of the brain and of great interest to the regenerative medicine field. It is complex and highly heterogeneous, including neural stem cells (NSCs) in different states of activation, rapid-amplifying progenitors, immature neuroblasts (NBs), mature neurons and other non-neurogenic populations. This chapter provides a step-by-step overview of a versatile flow cytometry-based protocol, which has been molecularly and functionally validated to classify and isolate the complete neurogenic lineage, including three NSC fractions (quiescent, primed, and activated), without the need for reporter mice. The panel is adaptable to diverse fluorescence needs and different cell targets, including niche differentiated cells such as endothelial cells, oligodendrocytes, or microglia, enabling the identification and isolation of the vast majority of cell types present in the SEZ. Additionally, it allows the study of cell cycling dynamics by means of 5-ethynyl-20-deoxyuridine (EdU) incorporation. The method enables the isolation of the different SEZ fractions and the functional assay of their cycling heterogeneity, including quiescence-activation transitions of NSC.
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