Evidence map›Paper›PMID 40067546›Full record

ArticleOdontology2025

Use of confocal laser scanning microscopy to evaluate the metal ion removal and destabilization of Enterococcus faecalis biofilms by EDTA and etidronic acid.

Talita Tartari, Carlos Estrela, Larissa Barbosa Borges de Araújo, Márcia Sirlene Zardin Graeff, Flaviana Bombarda de Andrade, Marco Antonio Hungaro Duarte

Abstract read
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In one paragraph

Article in Odontology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Talita TartariDepartment of Restorative Dentistry, Piracicaba School of Dentistry, State University of Campinas, Av. Limeira, 901, Piracicaba, SP, 13414-903, Brazil. talita_t@hotmail.com.ORCID http://orcid.org/0000-0003-4372-0158
Carlos EstrelaDepartment of Stomatology Sciences, Faculty of Dentistry, Federal University of Goias, Goiânia, GO, Brazil.ORCID http://orcid.org/0000-0002-1488-0366
Larissa Barbosa Borges de AraújoDepartment of Operative Dentistry, Endodontics, and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.ORCID http://orcid.org/0000-0003-1868-014X
Márcia Sirlene Zardin GraeffDepartment of Operative Dentistry, Endodontics, and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.ORCID http://orcid.org/0000-0002-6854-7913
Flaviana Bombarda de AndradeDepartment of Operative Dentistry, Endodontics, and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.ORCID http://orcid.org/0000-0002-1238-2160
Marco Antonio Hungaro DuarteDepartment of Operative Dentistry, Endodontics, and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.ORCID http://orcid.org/0000-0003-3051-737X

Funding

Fundação de Amparo à Pesquisa do Estado de São Paulo 2017/22364-5Fundação de Amparo à Pesquisa do Estado de São Paulo 2018/12690-5
6 · The paper itself

Abstract

Chelating substances bind to metals, forming stable complexes, rendering these essential ions unavailable for microbial metabolism and community stability in biofilms. This action can contribute to the disinfection in endodontic treatments. Through confocal laser scanning microscopy (CLSM), this study quantified the metal ion removal by chelating agents in Enterococcus faecalis biofilms and assessed the impact on community stability. E. faecalis biofilms were grown for 21 days on acrylic coverslips, which the following were immersed in (n = 10): G1) saline solution (control, 5 min); G2) 17% ethylenediaminetetraacetic acid (EDTA, 3 min); and G3) 9% etidronic acid (Dual Rinse HEDP, 5 min). Next, they were stained with Phen Green FL to identify metals, 4',6-diamidino-2-phenylindole (DAPI) to highlight bacterial DNA, and propidium iodide (PI) to mark nucleic acid in cells with damaged membranes. Images were captured (4/sample) by CLSM and analyzed by the software Leica Application Suite X. The individual and total volume of the biofilm-stained components (µm

Indexed as

BiofilmsChelating AgentsEdetic AcidEnterococcus faecalisEtidronic AcidMetalsMicroscopy, ConfocalChelating AgentsEdetic AcidEtidronic AcidMetalsBiofilmsChelating agentsFluorescent dyesMetalsMicroscopy, confocal

Identifiers

PMID40067546

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.