Evidence map›Paper›PMID 40067541›Full record

ArticleTissue engineering and regenerative medicine2025

Evaluation of Bi-layer Silk Fibroin Grafts for Onlay Urethroplasty in a Female Porcine Model of Long Urethral Strictures.

Gokhan Gundogdu, Travis Nguyen, David W Barham, Seyed Sharifi, Charlotte Morgan, Madison Rivero, Nuriel Sussman, Shubhang V Rajpara, Joel Gelman, Joshua R Mauney

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Tissue engineering and regenerative medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Gokhan GundogduDepartment of Urology, University of California, Irvine, Building 55, 101 The City Drive South., Rm. 300, Orange, CA, 92868, USA.
Travis NguyenDepartment of Biomedical Engineering, University of California, Irvine, Irvine, CA, 92617, USA.
David W BarhamDepartment of Urology, University of California, Irvine, Building 55, 101 The City Drive South., Rm. 300, Orange, CA, 92868, USA.
Seyed SharifiDepartment of Urology, University of California, Irvine, Building 55, 101 The City Drive South., Rm. 300, Orange, CA, 92868, USA.
Charlotte MorganDepartment of Urology, University of California, Irvine, Building 55, 101 The City Drive South., Rm. 300, Orange, CA, 92868, USA.
Madison RiveroDepartment of Biomedical Engineering, University of California, Irvine, Irvine, CA, 92617, USA.
Nuriel SussmanDepartment of Urology, University of California, Irvine, Building 55, 101 The City Drive South., Rm. 300, Orange, CA, 92868, USA.
Shubhang V RajparaDepartment of Biomedical Engineering, University of California, Irvine, Irvine, CA, 92617, USA.
Joel GelmanDepartment of Urology, University of California, Irvine, Building 55, 101 The City Drive South., Rm. 300, Orange, CA, 92868, USA.
Joshua R MauneyDepartment of Urology, University of California, Irvine, Building 55, 101 The City Drive South., Rm. 300, Orange, CA, 92868, USA. mauneyj@hs.uci.edu.ORCID http://orcid.org/0000-0002-3186-3664

Funding

Translational Bioreactors - Engineering Complex TissuesP41EB002520 · NIBIB · TUFTS UNIVERSITY MEDFORD · PI KAPLAN, DAVID L. · 2004 to 2018
$16.7M
Drug Eluting Silk Fibroin Grafts for Repair of Long Urethral StricturesR01DK131211 · NIDDK · UNIVERSITY OF CALIFORNIA-IRVINE · PI Joshua Robert Mauney · 2023 to 2026
$2.5M
NIBIB NIH HHS P41 EB002520NIDDK NIH HHS R01 DK131211NIDDK NIH HHS R01DK131211
6 · The paper itself

Abstract

backgroundBuccal mucosa urethroplasty represents the primary strategy for reconstruction of long urethral strictures (US). However, significant complications including stricture recurrence and donor site morbidity currently hamper this approach. The goal of this study was to determine the efficacy of acellular, bi-layer silk fibroin (BLSF) biomaterials to serve as superior alternatives to buccal mucosal (BM) grafts for repair of 4 cm long US in female swine.

methodsUrethral mucosal damage was induced over 4-5 cm long segments via electrocoagulation in adult female swine (N = 10) to promote US over the course of 2-4 weeks. Onlay urethroplasty with BLSF scaffolds or autologous BM grafts (N = 5 per group, ~ 4 cm

resultsAll swine survived the study with no severe complications and exhibited US formation following electrocoagulation with a 43-57% reduction in baseline calibers. At 3 months post-op, imaging modalities revealed both graft cohorts promoted > 80% restoration of native urethral calibers. Histological and IHC evaluations showed BLSF grafts supported the formation of innervated, vascularized urethral-like neotissues with α-smooth muscle actin + and SM22α + smooth muscle bundles as well as pan-cytokeratin + epithelia reminiscent of controls. In contrast, BM grafts primarily retained native oral tissue morphology after urethral transposition exhibiting cytokeratin 1 + stratified, squamous epithelia and scant muscle formation.

conclusionsBLSF matrices can promote functional restoration of long US via regeneration of native urethral tissues.

Indexed as

FibroinsUrethraUrethral StrictureAnimalsDisease Models, AnimalFemaleMouth MucosaSwineTissue ScaffoldsFibroinsBiomaterialSilk fibroinUrethral stricture disease

Identifiers

PMID40067541
PMCPMC11926291

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.