Construction and application of magnetic surface molecularly imprinted solid-phase extraction for the detection of 5-hydroxytryptamine in peripheral blood.

Gege Zhu, Xinyu Li, Qianjie Ma, Zhanying Hong, Dan Zhong, Xiaodong Sun

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Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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6 authors.

Gege Zhu *Shanghai Engineering Research Center of Organ Repair, Joint International Research Laboratory of Biomaterials and Biotechnology in Organ Repair (Ministry of Education), School of Medicine, Shanghai University, Shanghai, 200444, China.

Xinyu Li *Shanghai Engineering Research Center of Organ Repair, Joint International Research Laboratory of Biomaterials and Biotechnology in Organ Repair (Ministry of Education), School of Medicine, Shanghai University, Shanghai, 200444, China.

Qianjie MaShanghai Engineering Research Center of Organ Repair, Joint International Research Laboratory of Biomaterials and Biotechnology in Organ Repair (Ministry of Education), School of Medicine, Shanghai University, Shanghai, 200444, China.

Zhanying HongSchool of Pharmacy, Second Military Medical University, Shanghai, 200433, China. hongzhy001@163.com.

Dan ZhongShanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai, 200444, China. mzd_98@163.com.

Xiaodong SunShanghai Engineering Research Center of Organ Repair, Joint International Research Laboratory of Biomaterials and Biotechnology in Organ Repair (Ministry of Education), School of Medicine, Shanghai University, Shanghai, 200444, China. sunxiaodong_cpu@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The 5-Hydroxytryptamine (5-HT) level determination is crucial for predicting, pathogenesis, diagnosis, and pharmacological treatment of schizophrenia. To realize the extraction of trace 5-HT in complex matrix samples, a magnetic molecularly imprinted solid-phase extraction (MMISPE) pretreatment method was developed. In combination with UPLC-MS/MS, the method was possible to achieve the quantification of 1 ng/mL 5-HT in tissue samples. The type of adsorbent, magnetic surface molecularly imprinted polymers (MMIPs) adsorbent dosage, adsorption temperature, and adsorption method were screened to obtain the better extraction of 5-HT. After optimizing the extraction and separation method, we applied the MMISPE method to the detection of 5-HT in peripheral blood of clinical schizophrenia patients. The results showed that the average concentration of 5-HT in the peripheral blood of healthy controls was 74.30 ng/mL, whereas the 5-HT content in the samples from schizophrenic patients was 1.17 ng/mL.

Indexed as

Molecular ImprintingMolecularly Imprinted PolymersSerotoninSolid Phase ExtractionAdsorptionChromatography, High Pressure LiquidHumansLimit of DetectionMagnetic PhenomenaMaleSchizophreniaSurface PropertiesTandem Mass SpectrometryMolecularly Imprinted PolymersSerotonin5-HydroxytryptamineMolecularly imprinted polymersSchizophreniaSelective adsorptionUPLC-MS/MS

Identifiers

PMID40067417

DOI10.1007/s00604-025-07058-4

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.