ArticleThe Journal of clinical investigation2025
Proteostasis and metabolic dysfunction characterize a subset of storage-induced senescent erythrocytes targeted for posttransfusion clearance.
Article in The Journal of clinical investigation, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Blood-storage duration affects hematological and metabolic profiles in patients with sickle cell disease receiving transfusions.The Journal of clinical investigation · 2025Trial
- The Proteasome Safeguards Red Blood Cell Integrity During Storage and After Transfusion.Antioxidants (Basel, Switzerland) · 2026Article
- Curcumin's Protective Effects Against HMolecules (Basel, Switzerland) · 2026Review
- Improved red blood cell storage quality of blood from donors carrying the hypermorphic PIMT I120 variant.HemaSphere · 2026Article
- Lower efficacy of transfusion of red blood cells from donors with sickle cell trait.HemaSphere · 2026Article
- Genetic architecture of the murine red blood cell proteome reveals central role of hemoglobin beta cysteine 93 in maintaining redox balance.Cell genomics · 2026Article
- The "morning cup of joe" as a modifiable factor in blood transfusion.Haematologica · 2026Article
- Caffeine impairs red blood cell storage quality by dual inhibition of ADORA2b signaling and G6PD activity.Haematologica · 2026Article
- Longitudinal metabolomics study of phosphate-adenine-guanosine-glucose-saline-mannitol stored red blood cells.Transfusion · 2026Article
- Long-Distance Trail Running Induces Inflammatory-Associated Protein, Lipid, and Purine Oxidation in Red Blood Cells.Blood red cells & iron · 2026Article
- A novel AI-coupled flow chamber method quantifying erythrocyte osmotic fragility.Scientific reports · 2026Article
- Choice of lipid supplementation for in vitro erythroid cell culture impacts reticulocyte yield and characteristics.Scientific reports · 2026Article
- Ubiquitination and degradation of CD47 enhances macrophage phagocytosis of hemolytic erythrocytes.iScience · 2026Article
- Surface acoustic wave hemolysis assay for evaluating stored red blood cells.Lab on a chip · 2026Article
- Methyl eugenol attenuates age-associated oxidative fragility by coupling CaFrontiers in physiology · 2026Article
- HypomorphicHemaSphere · 2026Article
- GPX4 regulates lipid peroxidation and ferroptosis of stored red blood cells.Blood red cells & iron · 2025Article
- Supercooled storage of red blood cells slows down the metabolic storage lesion.Scientific reports · 2025Article
- From metabolomics to transfusion-associated immunomodulation.Current opinion in immunology · 2025Review
- Exposure to sub-zero temperatures down to -11 °C does not impact packed red cells storage quality.Scientific reports · 2025Article
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Abstract
Although refrigerated storage slows the metabolism of volunteer donor RBCs, which is essential in transfusion medicine, cellular aging still occurs throughout this in vitro process. Storage-induced microerythrocytes (SMEs) are morphologically altered senescent RBCs that accumulate during storage and are cleared from circulation following transfusion. However, the molecular and cellular alterations that trigger clearance of this RBC subset remain to be identified. Using a staining protocol that sorts long-stored SMEs (i.e., CFSEhi) and morphologically normal RBCs (CFSElo), these in vitro aged cells were characterized. Metabolomics analysis identified depletion of energy, lipid-repair, and antioxidant metabolites in CFSEhi RBCs. By redox proteomics, irreversible protein oxidation primarily affected CFSEhi RBCs. By proteomics, 96 proteins, mostly in the proteostasis family, had relocated to CFSEhi RBC membranes. CFSEhi RBCs exhibited decreased proteasome activity and deformability; increased phosphatidylserine exposure, osmotic fragility, and endothelial cell adherence; and were cleared from the circulation during human spleen perfusion ex vivo. Conversely, molecular, cellular, and circulatory properties of long-stored CFSElo RBCs resembled those of short-stored RBCs. CFSEhi RBCs are morphologically and metabolically altered, have irreversibly oxidized and membrane-relocated proteins, and exhibit decreased proteasome activity. In vitro aging during storage selectively alters metabolism and proteostasis in these storage-induced senescent RBCs targeted for clearance.
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