Evidence map›Paper›PMID 40064946›Full record

ArticleScientific reports2025

The role of Gln269Leu mutation on the thermostability and structure of uricase from Aspergillus flavus.

Mona Akhlaghi, Bagher Seyedalipour, Mohammad Pazhang, Mehdi Imani

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. AMB Express · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Mona AkhlaghiDepartment of Molecular and Cell Biology, Faculty of Basic Science, University of Mazandaran, Babolsar, Iran.
Bagher SeyedalipourDepartment of Molecular and Cell Biology, Faculty of Basic Science, University of Mazandaran, Babolsar, Iran. b.alipour81@gmail.com.
Mohammad PazhangDepartment of Biology, Faculty of Science, Azarbaijan Shahid Madani University, Tabriz, Iran.
Mehdi ImaniDepartment of Biochemistry, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Aspergillus flavus Urate oxidase (AFUOX) is promising for potential therapeutic applications, particularly in gout treatment. However, the enzyme's low thermostability and solubility limit its efficacy. A targeted mutation, substituting Gln with Leu at position 269 (Q269L) has been proposed to enhance its stability. The turnover number, catalytic efficiency, and specific activity of Q269L were 3.7 (s

Indexed as

Aspergillus flavusFungal ProteinsMutationUrate OxidaseEnzyme StabilityHydrogen-Ion ConcentrationKineticsMolecular Dynamics SimulationTemperatureThermodynamicsFungal ProteinsUrate OxidaseAspergillus flavusMolecular dynamics simulationQ269L mutantThermal stabilityUricase

Identifiers

PMID40064946
PMCPMC11894227

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.