Identification of Interaction Between Geminivirus and Plant Proteins by Bimolecular Fluorescence Complementation (BiFC).

Min Du, Xueping Zhou, Xiuling Yang

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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

3 authors.

Min DuState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

Xueping ZhouState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China. zzhou@zju.edu.cn.

Xiuling YangState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China. yangxiuling@caas.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Geminiviruses rely heavily on complicated protein-protein interactions between viral proteins and between virus and host plant proteins to establish successful infection. The bimolecular fluorescence complementation (BiFC) assay is based on the in vivo complementation of nonfluorescent component parts of a fluorescent protein through the interaction of proximity target proteins, each fused to a different part of the fluorescent protein. Here, we describe a protocol for the BiFC-based method for the identification of the interaction between geminivirus and plant proteins, which allows for the visualization of protein-protein interactions and the subcellular localization of protein interaction complexes in living systems.

Indexed as

GeminiviridaeHost-Pathogen InteractionsPlant ProteinsProtein Interaction MappingViral ProteinsLuminescent ProteinsMicroscopy, FluorescenceNicotianaProtein BindingLuminescent ProteinsPlant ProteinsViral ProteinsBimolecular fluorescence complementationConfocal microscopyGeminivirusProtein-protein interactionSubcellular localization

Identifiers

PMID40064785

DOI10.1007/978-1-4939-1261-2_11

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