ArticleMethods in molecular biology (Clifton, N.J.)2025
Tissue Printing Hybridization Using Digoxigenin-Labeled DNA Probes for the Detection of Geminiviruses and Associated DNA Satellites.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Nucleic acid hybridization is a fundamental technique in plant virus research, enabling precise detection of plant pathogens. Tissue printing hybridization exhibits high sensitivity and specificity in detecting viral genomes, providing particularly valuable for screening a large number of samples without the need for DNA purification. This method virtually eliminates contamination and false positives. This chapter presents a detailed and effective protocol for nucleic acid hybridization of geminiviruses using digoxigenin-labeled probes. Specifically, the tissue printing hybridization technique is outlined for a particular geminivirus and its associated deltasatellite. However, the methodology is adaptable to a diverse range of geminiviruses and host plants, rendering it versatile and applicable across various geminivirus research contexts.
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