Evidence map›Paper›PMID 40064427›Full record

ArticleThe Journal of steroid biochemistry and molecular biology2025

Artifactual elevations of 11β-hydroxyandrostenedione and 11-ketoandrostenedione in mass spectrometry assays.

Chaelin Lee, Patrick O'Day, David G Stouffer, Richard J Auchus, Adina F Turcu

Abstract read
In one paragraph

Article in The Journal of steroid biochemistry and molecular biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Trial
  2. Article
  3. Article
  4. Sex Hormones Associate With Amyotrophic Lateral Sclerosis Risk and Survival.Annals of clinical and translational neurology · 2026
    Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Chaelin LeeDivision of Metabolism, Endocrinology and Diabetes, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, United States.
Patrick O'DayDivision of Metabolism, Endocrinology and Diabetes, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, United States.
David G StoufferDivision of Metabolism, Endocrinology and Diabetes, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, United States.
Richard J AuchusDivision of Metabolism, Endocrinology and Diabetes, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, United States; Department of Pharmacology, University of Michigan, Ann Arbor, MI 48109, United States.
Adina F TurcuDivision of Metabolism, Endocrinology and Diabetes, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, United States. Electronic address: aturcu@umich.edu.

Funding

Activation of androgen biosynthesis and drug metabolism by cytochrome b5R01GM086596 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI RICHARD J. AUCHUS · 2009 to 2026
$4.8M
Primary Aldosteronism Subtypes: Pathophysiology and Steroid SignaturesR01HL155834 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI TURCU, ADINA F · 2021 to 2025
$3.5M
11-Oxyandrogens and Aging: Health ImplicationsR01AG080516 · NIA · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Adina F Turcu · 2023 to 2026
$2.3M
NHLBI NIH HHS R01 HL155834NIA NIH HHS R01 AG080516NIGMS NIH HHS R01 GM086596
6 · The paper itself

Abstract

Comprehensive steroid profiling by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) can be achieved using small biospecimen samples. LC-MS/MS assays offer superior accuracy to immunoassays, but they also introduce layers of complexity and opportunities for errors. Validation and harmonization studies are essential to ensure reliable results, as these assays are being increasingly incorporated in clinical laboratories. We developed an LC-MS/MS assay that simultaneously quantifies 18 Δ

Indexed as

AndrostenedioneTandem Mass SpectrometryArtifactsChromatography, LiquidCortisoneHumansHydrocortisone11-hydroxyandrostenedioneAndrostenedioneCortisoneHydrocortisone11-oxygenated androgen11β-hydroxyandrostenedioneAndrogenAssayCortisolCortisoneMass spectrometry

Identifiers

PMID40064427
PMCPMC12380235

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.