ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025
Wnt Inhibition Safeguards Porcine Embryonic Stem Cells From the Acquisition of Extraembryonic Endoderm Cell Fates.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed.
- Article
- Wnt/β-Catenin Activation by iCRT3 Enhanced the Pluripotency of Bovine Expanded Pluripotent Stem Cells.Animals : an open access journal from MDPI · 2026Article
- Advances in porcine stem cell research and their applications in agriculture.Frontiers in cell and developmental biology · 2026Review
- Overexpression of the lncRNA TUG1 regulates high-glucose-induced endocytic clearance dysfunction in HLSECs via the Wnt/β-catenin signaling pathway.European journal of medical research · 2025Article
- Beclin1 deficiency unlocks cardiac lineage commitment through convergent Wnt and BMP signaling activation.Stem cell research & therapy · 2025Article
- Wnt Inhibition Safeguards Porcine Embryonic Stem Cells From the Acquisition of Extraembryonic Endoderm Cell Fates.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Porcine embryonic stem cells (ESCs) are excellent models for exploring embryogenesis, producing genetically enhanced farm animals, and improving breeding. Various chemicals have been applied to generate porcine ESCs from embryos, which differ from mouse and human ESC derivation. Wnt inhibitors XAV939 or IWR1 are required to isolate and maintain porcine ESCs. How Wnt inhibitors specify porcine ESC fate decisions remains poorly understood. Additionally, whether porcine ESCs can be converted to extraembryonic endoderm (XEN) cells without genetic interventions has not been reported. Here, it is reported that Wnt inhibitors (i.e., XAV939 and IWR1) safeguard porcine ESCs from acquiring the XEN lineage. Porcine ESCs rely on Wnt inhibitors to maintain pluripotency. Without them, porcine ESCs exit from pluripotency and convert to XEN cells. An efficient strategy and culture conditions are further developed to directly derive porcine XEN cells from ESCs without gene editing. The resulting XEN cells from ESCs exhibit similar transcriptome and chromatin accessibility features to XEN cells from embryos and contribute to mouse extraembryonic tissues. This study will deepen the understanding of porcine pluripotency, lay the foundation for deriving high-quality porcine ESCs with germline chimerism and transmission, and provide valuable materials to study extraembryonic development and lineage segregation in livestock.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.