Evidence map›Paper›PMID 40063318›Full record

ArticleJournal of biomolecular NMR2025

Improvement in protein HSQC spectra from addition of betaine.

Finn O'Dea, Aiden J Seargeant, Jessica Hurcum, Rodolpho do Aido-Machado, Michelle L Rowe, Nicola J Baxter, Jon P Waltho, Jon R Sayers, Mike P Williamson

Abstract read
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In one paragraph

Article in Journal of biomolecular NMR, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Finn O'DeaSchool of Biosciences, University of Sheffield, Sheffield, S10 2TN, UK.
Aiden J SeargeantSchool of Biosciences, University of Sheffield, Sheffield, S10 2TN, UK.
Jessica HurcumSchool of Biosciences, University of Sheffield, Sheffield, S10 2TN, UK.
Rodolpho do Aido-MachadoSchool of Biosciences, University of Sheffield, Sheffield, S10 2TN, UK.ORCID http://orcid.org/0000-0001-6331-4216
Michelle L RoweSchool of Biosciences, University of Sheffield, Sheffield, S10 2TN, UK.
Nicola J BaxterSchool of Biosciences, University of Sheffield, Sheffield, S10 2TN, UK.
Jon P WalthoSchool of Biosciences, University of Sheffield, Sheffield, S10 2TN, UK.
Jon R SayersDivision of Clinical Medicine, School of Medicine and Population Health, University of Sheffield, Sheffield, S10 2RX, UK.ORCID http://orcid.org/0000-0002-5082-1443
Mike P WilliamsonSchool of Biosciences, University of Sheffield, Sheffield, S10 2TN, UK. m.williamson@sheffield.ac.uk.ORCID http://orcid.org/0000-0001-5572-1903

Funding

Bill and Melinda Gates Foundation NV-040070Department of Health and Social Care NIHR200636Gates Foundation INV-040070
6 · The paper itself

Abstract

Addition of glycine betaine up to 1 M gave rise to increased intensity for some weak signals in the HSQC spectra of barnase and Plasmodium falciparum flap endonuclease. The signals that were enhanced were low intensity signals, often from amide groups with rapid internal motion (low order parameter). The majority of signals are however somewhat weaker because of the increased viscosity. Addition of betaine is shown to cause a small but significant overall increase in order parameter, no consistent effect on conformational change on the µs-ms timescale, and a reduction in amide exchange rates, by a factor of about 3. The results are consistent with a model in which betaine leads to a reduction in fluctuations within the bulk water, which in turn produces a reduction in internal fluctuations of the protein.

Indexed as

BetaineNuclear Magnetic Resonance, BiomolecularProteinsRibonucleasesPlasmodium falciparumProtein ConformationBetaineProteinsRibonucleasesAmide exchangeBarnaseBetaineDynamicsFlap endonucleaseFluctuationOsmolyte

Identifiers

PMID40063318
PMCPMC12378501

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.