Evidence map›Paper›PMID 40060607›Full record

ArticlebioRxiv : the preprint server for biology2025

3D spatial transcriptomics reveals the molecular structure of input and output pathways in the mouse olfactory bulb.

Nell Klimpert, Mihaly Kollo, David H Brann, Chichun Tan, David Barry, Ying Ma, Andreas T Schaefer, Alexander Fleischmann

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Nell KlimpertDepartment of Neuroscience and Carney Institute for Brain Science, Brown University, Providence, RI, USA.
Mihaly KolloSensory Circuits and Neurotechnology Laboratory, The Francis Crick Institute, London, UK.
David H BrannDepartment of Neurobiology, Harvard Medical School, Boston, MA, USA.
Chichun TanDepartment of Biostatistics and Center for Computational Molecular Biology, Brown University, Providence, RI, USA.
David BarryCrick Advanced Light Microscopy, The Francis Crick Institute, London, UK.ORCID 0000-0003-2763-5244
Ying MaDepartment of Biostatistics and Center for Computational Molecular Biology, Brown University, Providence, RI, USA.
Andreas T SchaeferSensory Circuits and Neurotechnology Laboratory, The Francis Crick Institute, London, UK.ORCID 0000-0002-4677-8788
Alexander FleischmannDepartment of Neuroscience and Carney Institute for Brain Science, Brown University, Providence, RI, USA.

Funding

The Structure of Olfactory Neural and Perceptual SpacesU19NS112953 · NINDS · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI DATTA, SANDEEP R · 2019 to 2023
$18.4M
Interdisciplinary Predoctoral Neuroscience Training Program in the Neuroscience Graduate Program.T32MH020068 · NIMH · BROWN UNIVERSITY · PI LIPSCOMBE, DIANE, SHEINBERG, DAVID L · 1999 to 2025
$7.6M
Brain Science Compute ClusterS10OD025181 · OD · BROWN UNIVERSITY · PI SANES, JEROME N · 2019 to 2019
$600k
NIH HHS S10 OD025181NIMH NIH HHS T32 MH020068NINDS NIH HHS U19 NS112953Wellcome Trust FC001153
6 · The paper itself

Abstract

A core organizing principle of the vertebrate brain is its symmetry along multiple axes. However, the precision with which neurons, circuit modules, and brain regions align to these axes remains poorly understood. Here, we used 3D spatial transcriptomics to reconstruct the anatomical and molecular organization of the mouse olfactory bulb. We mapped the positions of nearly one thousand molecularly distinct glomeruli, the structural and functional units of odor processing, revealing highly symmetric organization across hemispheres. Within each bulb, we delineated a curved axis of symmetry that divides pairs of sister glomeruli. Gene expression in the olfactory epithelium predicted glomerular position with near-glomerular resolution. However, glomerular symmetry did not extend to deeper layer mitral and granule cells, suggesting a reorganization from sensory input to cortical output pathways. Our findings provide the first comprehensive map of the olfactory bulb and reveal how its molecular structure is instructed by epithelial gene expression programs.

Indexed as

3D spatial transcriptomicsbrain symmetrygene expression programsinput-output organizationodorant receptorOlfactory circuits

Identifiers

PMID40060607
PMCPMC11888228

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.