ArticlebioRxiv : the preprint server for biology2025
Blue-shifted ancyromonad channelrhodopsins for multiplex optogenetics.
Elena G Govorunova, Oleg A Sineshchekov, Hai Li, Yueyang Gou, Hongmei Chen, Shuyuan Yang, Yumei Wang, Stephen Mitchell, Alyssa Palmateer, Leonid S Brown and 3 more
Abstract readPreprint
In one paragraphArticle in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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4 · The recordCorrections and comments
5 · Who and what moneyAuthors and funding
13 authors.
Elena G GovorunovaCenter for Membrane Biology, Department of Biochemistry & Molecular Biology, The University of Texas Health Science Center at Houston McGovern Medical School, Houston, TX 77030, USA.ORCID 0000-0003-0522-9683 Oleg A SineshchekovCenter for Membrane Biology, Department of Biochemistry & Molecular Biology, The University of Texas Health Science Center at Houston McGovern Medical School, Houston, TX 77030, USA.
Hai LiCenter for Membrane Biology, Department of Biochemistry & Molecular Biology, The University of Texas Health Science Center at Houston McGovern Medical School, Houston, TX 77030, USA.ORCID 0000-0002-3969-6709 Yueyang GouDepartment of Neuroscience, Baylor College of Medicine; Houston, TX 77030, USA.
Hongmei ChenDepartment of Neuroscience, Baylor College of Medicine; Houston, TX 77030, USA.
Shuyuan YangDepartment of Chemical and Biomolecular Engineering, Rice University, Houston, TX 77005, USA.
Yumei WangCenter for Membrane Biology, Department of Biochemistry & Molecular Biology, The University of Texas Health Science Center at Houston McGovern Medical School, Houston, TX 77030, USA.
Stephen MitchellDepartment of Physics and Biophysics Interdepartmental Group, University of Guelph; Guelph, Ontario N1G 2W1, Canada.
Alyssa PalmateerDepartment of Physics and Biophysics Interdepartmental Group, University of Guelph; Guelph, Ontario N1G 2W1, Canada.
Leonid S BrownDepartment of Physics and Biophysics Interdepartmental Group, University of Guelph; Guelph, Ontario N1G 2W1, Canada.
John L SpudichCenter for Membrane Biology, Department of Biochemistry & Molecular Biology, The University of Texas Health Science Center at Houston McGovern Medical School, Houston, TX 77030, USA.ORCID 0000-0003-4167-8590 Funding
Molecular Engineering of Natural Light-Gated Chloride Channels for Optogenetic InhibitionU01NS118288 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI SPUDICH, JOHN LEE, ST-PIERRE, FRANCOIS · 2020 to 2023
$5.2MDeveloping an Optogenetics Technology Based on Natural Potassium-selective ChannelrhodopsinsRF1NS133657 · NINDS · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI SPUDICH, JOHN LEE, ST-PIERRE, FRANCOIS · 2023 to 2023
$3.1MStructure/Function of Channelrhodopsins and Related Retinylidene ProteinsR35GM140838 · NIGMS · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI SPUDICH, JOHN LEE · 2021 to 2025
$3.1MRed-shifted voltage indicators for two-photon all-optical physiology and multi-spectral imagingR01NS136027 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI Jacob Reimer, Francois St-Pierre · 2024 to 2026
$1.7MA multiplexed high-throughput platform to report pharmacologic alteration of cancer stem cell membrane potential and cell cycle stateR61CA278458 · NCI · METHODIST HOSPITAL RESEARCH INSTITUTE · PI ROBERT C ROSTOMILY, Francois St-Pierre · 2024 to 2026
$686kHigh-Throughput Automated Patch Clamp SystemS10OD032293 · OD · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI SPUDICH, JOHN LEE · 2022 to 2022
$599kNCI NIH HHS R61 CA278458NIGMS NIH HHS R35 GM140838NIH HHS S10 OD032293NINDS NIH HHS R01 NS136027NINDS NIH HHS RF1 NS133657NINDS NIH HHS U01 NS118288
6 · The paper itselfAbstract
Light-gated ion channels from protists (channelrhodopsins or ChRs) are optogenetic tools widely used for controlling neurons and cardiomyocytes. Multiplex optogenetic applications require spectrally separated molecules, which are difficult to engineer without disrupting channel function. Scanning numerous sequence databases, we identified three naturally blue-shifted ChRs from ancyromonads. They form a separate branch on the phylogenetic tree and contain residue motifs characteristic of anion ChRs (ACRs). However, only two conduct chloride, whereas the closely related
Identifiers
PMID40060592
PMCPMC11888301
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