Evidence map›Paper›PMID 40060436›Full record

ArticlebioRxiv : the preprint server for biology2025

Lamin B loss in nuclear blebs is rupture dependent while increased DNA damage is rupture independent.

Catherine G Chu, Nick Lang, Erin Walsh, Mindy D Zheng, Gianna Manning, Kiruba Shalin, Lyssa M Cunha, Kate E Faucon, Nicholas Kam, Sara N Folan and 13 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

23 authors.

Catherine G ChuBiology department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Nick LangBiology department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Erin WalshBiology department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Mindy D ZhengBiology department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Gianna ManningBiology department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Kiruba ShalinBiology department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Lyssa M CunhaBiology department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Kate E FauconBiology department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Nicholas KamBiology department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Sara N FolanBiology department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Arav P DesaiBiology department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Emily NaughtonBiology department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Jaylynn AbreuBiology department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Alexis M CarsonBiology department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Zachary L WaldBiology department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Dasha Khvorova-WolfsonBiology department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Leena PhanBiology department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Hannah LeeBiology department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Mai PhoBiology department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Kelsey PrinceBiology department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Katherine DorfmanBiology department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Michael Seifu BahiruBiology department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Andrew D StephensBiology department, University of Massachusetts Amherst, Amherst, MA 01003, USA.ORCID 0000-0001-5474-7845

Funding

Role of chromatin mechanics in nuclear shape and integrityR35GM154928 · NIGMS · UNIVERSITY OF MASSACHUSETTS AMHERST · PI Andrew Daniel Stephens · 2024 to 2026
$1.2M
NIGMS NIH HHS R35 GM154928
6 · The paper itself

Abstract

The nucleus houses genetic information and functions separate from the rest of the cell. Loss of nuclear shape results in nuclear ruptures. Nuclear blebs are deformations identified by decreased DNA density, while lamin B levels vary drastically. To determine if decreased lamin B levels are due to nuclear rupture, we used immunofluorescence to measure levels of lamin B and emerin, a nuclear envelope protein that enriches to sites of nuclear rupture. We observed that cell types that exhibit decreased levels of lamin B also show an enrichment of emerin in nuclear blebs. Oppositely, in other cell types, nuclear blebs display maintained levels of lamin B1 and showed no emerin enrichment. To determine how nuclear rupture affects DNA damage, we time lapse imaged nuclear rupture dynamics then fixed the same cells to conduct immunofluorescence of γH2AX and emerin. We find that DNA damage levels are higher in blebbed nuclei independent of nuclear rupture. Thus, we confirm that lamin B1 loss in nuclear blebs is due to nuclear rupture and blebbed nuclei have increased DNA damage that is independent of rupture.

Indexed as

DNA damageemerinlamin Bnuclear blebnuclear rupturenucleus

Identifiers

PMID40060436
PMCPMC11888350

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.