Evidence map›Paper›PMID 40060183›Full record

ArticleaBIOTECH2025

A high-throughput protocol for testing heat-stress tolerance in pollen.

Chenchen Zhao, Abu Bakar Siddique, Ce Guo, Sergey Shabala, Chengdao Li, Zhonghua Chen, Rajeev Varshney, Meixue Zhou

Abstract read
In one paragraph

Article in aBIOTECH, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Chenchen ZhaoTasmanian Institute of Agriculture, University of Tasmania, Prospect, TAS 7250 Australia.
Abu Bakar SiddiqueTasmanian Institute of Agriculture, University of Tasmania, Prospect, TAS 7250 Australia.
Ce GuoTasmanian Institute of Agriculture, University of Tasmania, Prospect, TAS 7250 Australia.
Sergey ShabalaTasmanian Institute of Agriculture, University of Tasmania, Prospect, TAS 7250 Australia.
Chengdao LiFood Futures Institute, School of Agriculture, Western Crop Genetics Alliance, Murdoch University, Murdoch, WA 6150 Australia.
Zhonghua ChenSchool of Science, Western Sydney University, Penrith, NSW 2751 Australia.
Rajeev VarshneyFood Futures Institute, School of Agriculture, Western Crop Genetics Alliance, Murdoch University, Murdoch, WA 6150 Australia.
Meixue ZhouTasmanian Institute of Agriculture, University of Tasmania, Prospect, TAS 7250 Australia.ORCID 0000-0003-3009-7854

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Viable pollen is crucial for fertilization, but pollen is generally highly susceptible to heat stress. A quick, reliable method for testing the heat-stress tolerance of pollen is needed to improve the heat-stress tolerance in plants, but current methods require considerable space and labor. In addition, many such methods only test tolerance to a single constant temperature, making it time-consuming to screen heat tolerance over a wide temperature range and to examine the dynamics of pollen viability at different temperatures. To address this issue, we aimed to: (1) develop an easy, reliable method for measuring pollen viability at different temperatures; and (2) identify the best temperature range for screening pollen with high heat-stress tolerance. We harvested mature pollen from wheat ( Supplementary Information: The online version contains supplementary material available at 10.1007/s42994-024-00183-3.

Indexed as

Heat stressHordeum vulgare L.Pollen germinationPollen tube developmentTriticum aestivum L.

Identifiers

PMID40060183
PMCPMC11889306

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.