Evidence map›Paper›PMID 40060182›Full record

ArticleaBIOTECH2025

Convenient, high-efficiency multiplex genome editing in autotetraploid alfalfa using endogenous

Xiuzhi Xia, Shihao Li, Na Wang, Panxu Cheng, Butuo Zhu, Pengcheng Zhang, Dahai Yang, Hao Lin, Lifang Niu

Abstract read
In one paragraph

Article in aBIOTECH, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Advances in CRISPR/Cas9-Based Gene Editing in Filamentous Fungi.Journal of fungi (Basel, Switzerland) · 2025
    Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Xiuzhi Xia *Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.
Shihao Li *Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.
Na WangBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.
Panxu ChengBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.
Butuo ZhuBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.
Pengcheng ZhangBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.
Dahai YangTobacco Breeding and Biotechnology Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Kunming, 650021 China.
Hao LinBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.ORCID 0000-0003-4717-8335
Lifang NiuBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

CRISPR/Cas is a simple, robust, versatile tool for plant biology studies and precision plant breeding. However, establishing a high-efficiency gene editing system for multiplex editing of the autotetraploid crop alfalfa ( Supplementary Information: The online version contains supplementary material available at 10.1007/s42994-025-00200-z.

Indexed as

AlfalfaHairy root systemMsU6 promoterMultiplex genome editingVisual reporters

Identifiers

PMID40060182
PMCPMC11889276

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.