Evidence map›Paper›PMID 40059311›Full record

ArticleBiomacromolecules2025

Controlling Protein Immobilization over Poly(3-hydroxybutyrate) Microparticles Using Substrate Binding Domain from PHA Depolymerase.

Isabela P Dias, Regiane Stafim da Cunha, Ryu Masaki, Maritza A Todo Bom, Edneia A S Ramos, Giovanna J V P Dos Santos, Giovanna Furman, Julia T Lucena, Isabella G Jiacomini, Sze M Lo and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomacromolecules, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Isabela P DiasDepartment of Biochemistry, Federal University of Paraná, Curitiba 80060-000, PR, Brazil.
Regiane Stafim da CunhaDepartment of Basic Pathology, Federal University of Paraná, Curitiba 80060-000, PR, Brazil.
Ryu MasakiDepartment of Biochemistry, Federal University of Paraná, Curitiba 80060-000, PR, Brazil.
Maritza A Todo BomDepartment of Biochemistry, Federal University of Paraná, Curitiba 80060-000, PR, Brazil.
Edneia A S RamosDepartment of Basic Pathology, Federal University of Paraná, Curitiba 80060-000, PR, Brazil.
Giovanna J V P Dos SantosDepartment of Genetics, Evolution, Microbiology and Immunology, University of Campinas, Campinas 13083-970, SP, Brazil.
Giovanna FurmanDepartment of Biochemistry, Federal University of Paraná, Curitiba 80060-000, PR, Brazil.
Julia T LucenaDepartment of Biochemistry, Federal University of Paraná, Curitiba 80060-000, PR, Brazil.
Isabella G JiacominiDepartment of Basic Pathology, Federal University of Paraná, Curitiba 80060-000, PR, Brazil.
Sze M LoDepartment of Basic Pathology, Federal University of Paraná, Curitiba 80060-000, PR, Brazil.
Zelinda Schemczssen-GraeffDepartment of Basic Pathology, Federal University of Paraná, Curitiba 80060-000, PR, Brazil.
Breno C B BeirãoDepartment of Basic Pathology, Federal University of Paraná, Curitiba 80060-000, PR, Brazil.
Silvio M ZanataDepartment of Basic Pathology, Federal University of Paraná, Curitiba 80060-000, PR, Brazil.
Luiz M de L FariaDepartment of Chemistry and Biology, Federal Technological University of Paraná, Curitiba 81531-980, PR, Brazil.
Edileusa M GerhardtDepartment of Biochemistry, Federal University of Paraná, Curitiba 80060-000, PR, Brazil.
Emanuel Maltempi de SouzaDepartment of Biochemistry, Federal University of Paraná, Curitiba 80060-000, PR, Brazil.
Marcelo Müller-SantosDepartment of Biochemistry, Federal University of Paraná, Curitiba 80060-000, PR, Brazil.ORCID 0000-0001-9615-6007
Guilherme F PichethDepartment of Biochemistry, Federal University of Paraná, Curitiba 80060-000, PR, Brazil.ORCID 0000-0002-2802-0180

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Biointerface decoration with ligands is a crucial requirement to modulate biodistribution, increase half-life, and provide navigation control for targeted micro- or nanostructured systems. To better control the process of ligand functionalization over three-dimensional (3D) polyester surfaces, we report the characterization of hybrid proteins developed to enhance the anchoring efficiency over polymeric surfaces and preserve optimal spatial orientation: sfGFP, mRFP1, and the RBD proteins were attached to a polyester substrate binding domain (SBD) formed by the C-terminus region of PHA depolymerase. The binding ability was evaluated over poly(3-hydroxybutyrate) (PHB) microparticles (MP) and two-dimensional (2D) surfaces. The PHB interfaces revealed a high affinity toward the proteins linked with SBD, displaying higher protein contents compared to untagged proteins. The MP decorated with RBD-SBD exhibited limited MRC5 internalization and cytotoxicity without a significant impact caused by the RBD protein, suggesting that the system might be adapted for targeted drug delivery and vaccine applications.

Indexed as

Carboxylic Ester HydrolasesEnzymes, ImmobilizedHydroxybutyratesPolyestersGreen Fluorescent ProteinsHumansPolyhydroxybutyratesProhibitinsProtein DomainsCarboxylic Ester HydrolasesEnzymes, ImmobilizedGreen Fluorescent ProteinsHydroxybutyratesPHB protein, humanpoly-beta-hydroxybutyratepoly-beta-hydroxybutyrate depolymerasePolyestersPolyhydroxybutyratesProhibitins

Identifiers

PMID40059311
PMCPMC12004514

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.