Evidence map›Paper›PMID 40059184›Full record

ArticleJournal of orthopaedic surgery and research2025

Ghrelin increases cis-platinum resistance and promotes aggressiveness of osteosarcoma by activating AKT and Wnt/β-catenin pathways.

Menglin Cong, Shufeng Li, Yu Fu, Ting Wang, Huizhen Wei, Xiaojie Ma, Fangming Liu, XieHuang Sheng, Kehan Long, Haocheng Hou and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of orthopaedic surgery and research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Menglin Cong *Department of Spine Surgery, Qilu Hospital of Shandong University, Jinan, China.
Shufeng Li *Department of Orthopedic Surgery, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, China.
Yu Fu *Department of Orthopedic Surgery, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, China.
Ting WangDepartment of Obstetrics and Gynecology, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, China.
Huizhen WeiDepartment of Obstetrics and Gynecology, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, China.
Xiaojie MaCollege of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan, China.
Fangming LiuDepartment of Rheumatology and immunology, Shandong Provincial Qianfoshan Hospital, Institute of Anesthesia and Respiratory Critical Medicine, The First Affiliated Hospital of Shandong First Medical University, Jinan, China.
XieHuang ShengCollege of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, China.
Kehan LongClinical Medical College of Weifang Medical University, Weifang, China.
Haocheng HouCollege of Basic Medicine, Shandong University, Jinan, China.
Xuecheng SunDepartment of Orthopedic Trauma, Weifang People's Hospital, Weifang, China. wfsunxuecheng@126.com.
Weiwei LiDepartment of Pathology, Qilu Hospital of Shandong University, Jinan, China. liweiweizhao@163.com.
Lei ZhangDepartment of Orthopedic Surgery, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, China. qygkzl1818@163.com.

Funding

National Natural Science Foundation of China 82073437
6 · The paper itself

Abstract

Osteosarcoma (OS) is the most common primary bone malignancy because of its extra high tendency of metastasis. In-depth research is needed to uncover the pathogenesis of patients with OS cells. We collected 74 tissue samples from patients with OS cells and measured the expression levels of ghrelin by immunohistochemistry. Ghrelin was added into OS cell lines in CCK8 assays, JC-1 staining and Western blot analysis were performed to explore its effect on the aggressiveness of OS cells and drug resistance. To determine its function, ghrelin was overexpressed or knocked down in OS cells and then detect cell proliferation in the xenograft mouse model and orthotopic model. Western blot analysis was performed to explore ghrelin-regulated signal pathways. In this work, we identified the relation between the level of ghrelin expression and poor prognosis of OS patients. As well as promoting proliferation, migration, and invation, ghrelin promotes the survival of OS in vitro as well as in vivo, and reduces the apoptosis of OS cells. What's more, ghrelin increases the resistance of cis-platinum by changing mitochondrial function and decreases the expression of MDR-1. Above all, these results demonstrated ghrelin exerts tumorigenic and metastatic effects and may be a potential therapeutic target.

Indexed as

Bone NeoplasmsCisplatinDrug Resistance, NeoplasmGhrelinOsteosarcomaProto-Oncogene Proteins c-aktWnt Signaling PathwayAnimalsApoptosisCell Line, TumorCell MovementCell ProliferationFemaleHumansMaleMiceCisplatinGhrelinProto-Oncogene Proteins c-aktAKT pathwayDrug-resistenceGhrelinOsteosarcomaWnt/β-catenin pathway

Identifiers

PMID40059184
PMCPMC11892314

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.