Evidence map›Paper›PMID 40059177›Full record

ArticleGut pathogens2025

Molecular confirmation of Cryptosporidium and Cyclospora species in children with acute diarrhoea in Quindio region, Colombia.

Jessica Triviño-Valencia, Alejandro Nati-Castillo, Nancy Yhomara Cabeza, Fabiana Lora-Suarez, Jorge Gómez-Marín

Erratum issuedAbstract read
In one paragraph

Article in Gut pathogens, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Rodent-Borne Parasites and Human Disease: A Growing Public Health Concern.Animals : an open access journal from MDPI · 2025
    Review
  4. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Jessica Triviño-ValenciaGrupo GEPAMOL, Centro de Investigaciones Biomédicas, Facultad de Ciencias de la Salud, Universidad del Quindío, Armenia, Quindío, Colombia.ORCID http://orcid.org/0000-0003-2447-2312
Alejandro Nati-CastilloGrupo de Investigación Clínica, Hospital Universitario San Juan de Dios, Armenia, Quindío, Colombia.ORCID http://orcid.org/0000-0003-1890-4983
Nancy Yhomara CabezaGrupo de Investigación Clínica, Hospital Universitario San Juan de Dios, Armenia, Quindío, Colombia.ORCID http://orcid.org/0000-0003-1336-7023
Fabiana Lora-SuarezGrupo GEPAMOL, Centro de Investigaciones Biomédicas, Facultad de Ciencias de la Salud, Universidad del Quindío, Armenia, Quindío, Colombia.ORCID http://orcid.org/0000-0003-0094-3044
Jorge Gómez-MarínGrupo GEPAMOL, Centro de Investigaciones Biomédicas, Facultad de Ciencias de la Salud, Universidad del Quindío, Armenia, Quindío, Colombia. jegomez@uniquindio.edu.co.ORCID http://orcid.org/0000-0001-6472-3329

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThere are no reports with molecular confirmation of Cryptosporidium spp. and Cyclospora spp. in children consulting the emergency service due to diarrhoea in Colombia.

methodsA descriptive study was performed on 137 children who visited the Hospital San Juan de Dios Emergency Service in Armenia between April 1 and 31, 2022. Questionnaires and sampling were performed to identify parasites in the faecal samples. Fresh preparations were prepared with 1% iodine, and a modified Ziehl-Neelsen stain was used to identify pathogenic intestinal protozoa (Cryptosporidium spp. and Cyclospora spp.). PCR and sequencing of positive samples were performed to confirm infection.

resultsThe prevalence of Cryptosporidium spp. infection in children was 19,7%, and that of Cyclospora spp. was 10,9%. 59,2% of the children with cryptosporidiosis and 66,6% of the children with cyclosporiasis were hospitalized. PCR for Cryptosporidium spp. was positive in six of 28 (21%) samples and for Cyclospora in 11 of 15 (73%) samples. Cyclospora spp. SSU rRNA DNA sequences clustered 10 samples nearest to lineage A, two with lineage B, and one with lineage C.

conclusionsCryptosporidiosis and cyclosporiasis are common in children with acute diarrhoea when consulting emergency services, and their search should be performed systematically.

Indexed as

ChildColombiaCryptosporidiosisCryptosporidiumCyclosporaCyclosporiasisDiarrheaPolymerase chain reactionPrevalenceProtozoa

Identifiers

PMID40059177
PMCPMC11892127

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.