Large-scale fermentation of Lactiplantibacillus pentosus 292 for the production of lactic acid and the storage strategy based on molasses as a preservative.

backgroundLactiplantibacillus pentosus 292 is a lactic acid bacterium (LAB) with significant probiotic potential, but large-scale production is often limited by high production costs and preservation challenges. This study aimed to develop a cost-effective medium to enhance lactic acid production and establish a feasible preservation strategy to support the strain's large-scale application.

resultsA low-cost medium containing glucose, yeast powder, K₂HPO₄, and Tween-80 was formulated, enabling Lactiplantibacillus pentosus 292 to achieve a lactic acid yield of 16.24 g/L, representing an 83.48% increase compared to the traditional MRS medium. Fermentation kinetics models for bacterial growth, substrate consumption, and product generation were established in a 200-L fermenter using the Logistic, Luedeking-Piret-like, and Luedeking-Piret models, and the R

conclusionIn this work, a cost-effective medium that significantly increased lactic acid yield and a preservative based on molasses as a strategy to extend the storage period of fermentation products were developed for large-scale production of L. pentosus 292, a member of probiotic LAB. Additionally, large-scale fermentation kinetics models were constructed, providing valuable technical insights for the large-scale production and application of this LAB, highlighting its significant potential for industrial applications.