Evidence map›Paper›PMID 40057673›Full record

ArticleBMC microbiology2025

Involvement of Mycobacterium smegmatis small noncoding RNA B11 in triacylglycerol accumulation and altered cell wall permeability.

Zhuhua Wu, Weilong Liu, Qiuchan Tan, Yuhui Chen, Xiaoyu Lai, Jianming Hong, Hongdi Liang, Huizhong Wu, Jing Liang, Xunxun Chen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. A Case of Transmission of Bedaquiline- and Linezolid-Resistant Tuberculosis.International journal of molecular sciences · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Zhuhua Wu *Key Laboratory of Translational Medicine of Guangdong, Center for Tuberculosis Control of Guangdong Province, Guangzhou, Guangdong, China.
Weilong Liu *Institute of Hepatology, Shenzhen Third People's Hospital, Shenzhen, Guangdong, China.
Qiuchan TanSchool of Basic Medical Sciences, Guangzhou Health Science College, Guangzhou, Guangdong, China.
Yuhui ChenKey Laboratory of Translational Medicine of Guangdong, Center for Tuberculosis Control of Guangdong Province, Guangzhou, Guangdong, China.
Xiaoyu LaiKey Laboratory of Translational Medicine of Guangdong, Center for Tuberculosis Control of Guangdong Province, Guangzhou, Guangdong, China.
Jianming HongThe Sixth People's Hospital of Dongguan, Dongguan, Guangdong, China.
Hongdi LiangKey Laboratory of Translational Medicine of Guangdong, Center for Tuberculosis Control of Guangdong Province, Guangzhou, Guangdong, China.
Huizhong WuKey Laboratory of Translational Medicine of Guangdong, Center for Tuberculosis Control of Guangdong Province, Guangzhou, Guangdong, China. 1627639699@qq.com.
Jing LiangThe Sixth People's Hospital of Dongguan, Dongguan, Guangdong, China. 13999641@qq.com.
Xunxun ChenKey Laboratory of Translational Medicine of Guangdong, Center for Tuberculosis Control of Guangdong Province, Guangzhou, Guangdong, China. grace_chen514@163.com.

Funding

Dongguan Science and Technology of Social Development Program 20211800904782Guangdong Provincial Clinical Research Center for Tuberculosis 2020B1111170014the Science and Technology Planning Project of Guangdong Province 2021B1212030003the Science and Technology Program of Guangzhou, China 202201011764the Special Fund for Science and technology innovation strategy of Guangdong province pdjh2024b632the Youth Innovative Talents Project in Colleges and Universities in Guangdong Province 2024KQNCX209
6 · The paper itself

Abstract

backgroundPathways involving triacylglycerol (TAG) accumulation are thought to play a crucial regulatory role in bacterial growth and metabolism. Despite this understanding, little is known about the biological functions and regulatory mechanisms of small RNAs in Mycobacterium. Mycobacterium smegmatis (M. smegmatis), a type of Mycobacterium, serves as a model organism to investigate the molecular, physiological, and drug resistance features of M. tuberculosis.

resultsIn this study, we demonstrated that overexpression of B11 significantly affects bacterial growth and colony morphology, increases antibiotic sensitivity and sodium dodecyl sulfate (SDS) surface stress, decreases intracellular survival, and suppresses cytokine secretion in macrophages. Transcriptomic and lipidomic analyses revealed a metabolic downshift in the B11 overexpression strain, characterized by reduced levels of TAG. Furthermore, transmission electron microscopy showed that the B11 overexpression strain exhibited decreased cell wall thickness, leading to reduced biofilm formation and altered cell wall permeability. Additionally, we observed that B11 regulated certain target genes but did not directly bind to those proteins tested.

conclusionsTaken together, these findings suggest that B11 plays important roles in Mycobacterium survival under antibiotic and SDS stresses, TAG accumulation, and contributes to antibiotic sensitivity through altered cell wall permeability.

Indexed as

Cell WallMycobacterium smegmatisRNA, BacterialRNA, Small UntranslatedTriglyceridesAnimalsAnti-Bacterial AgentsBiofilmsGene Expression Regulation, BacterialMacrophagesPermeabilitySodium Dodecyl SulfateAnti-Bacterial AgentsRNA, BacterialRNA, Small UntranslatedSodium Dodecyl SulfateTriglyceridesB11BiofilmsCell wall permeabilityMycobacterium smegmatisTriacylglycerol

Identifiers

PMID40057673
PMCPMC11889869

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.