Evidence map›Paper›PMID 40056408›Full record

ArticleSTAR protocols2025

Protocol to identify transcription factor target genes using TargetOrtho2.

Jonathan D Rumley, Jee Hun Kim, Oliver Hobert

Abstract read
In one paragraph

Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Jonathan D RumleyDepartment of Biological Sciences, Columbia University, New York, NY, USA; Howard Hughes Medical Institute, New York, NY 10027, USA. Electronic address: jdr2203@columbia.edu.
Jee Hun KimDepartment of Biological Sciences, Columbia University, New York, NY, USA.
Oliver HobertDepartment of Biological Sciences, Columbia University, New York, NY, USA; Howard Hughes Medical Institute, New York, NY 10027, USA. Electronic address: or38@columbia.edu.

Funding

Regulatory Circuitry and Mechanisms Controlling Cell Fate in C. elegansR35GM131746 · NIGMS · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI Iva S Greenwald · 2019 to 2026
$5.0M
Dissecting regulation and function of unc-6/Netrin via the generation of cis-regulatory allelesF32MH136667 · NIMH · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI Jonathan Douglas Rumley · 2024 to 2026
$243k
NIGMS NIH HHS R35 GM131746NIMH NIH HHS F32 MH136667
6 · The paper itself

Abstract

TargetOrtho2 uses transcription factor binding site information to predict transcription factor targets in C. elegans, based on an in silico phylogenetic footprinting approach. Here, we present a protocol to identify transcription factor target genes using a new version of TargetOrtho2. We provide instructions for installing TargetOrtho2 and its required suite of programs, for predicting transcription factor target genes, and for updating and adding new genomes to TargetOrtho2.

Indexed as

Caenorhabditis elegansComputational BiologySoftwareTranscription FactorsAnimalsBinding SitesCaenorhabditis elegans ProteinsPhylogenyCaenorhabditis elegans ProteinsTranscription FactorsBioinformaticsGene ExpressionGenomicsModel OrganismsMolecular BiologySequence analysis

Identifiers

PMID40056408
PMCPMC11930065

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.