Evidence map›Paper›PMID 40056293›Full record

ArticlePlant molecular biology2025

Disruption of the endoplasmic reticulum-localized fatty acyl-ACP thioesterase IPF1 caused partial male sterility in rice.

Wenye Tan, Jingfei Tian, Wenfeng Zhao, Jianxin Wei, Yibo Xu, Shixu Zhou, Zihan Wei, Zejun Shen, Minghang Wu, Lianguang Shang and 3 more

Abstract read
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In one paragraph

Article in Plant molecular biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Wenye Tan *State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Agricultural College, Guangxi University, Nanning, 530005, China.
Jingfei Tian *State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Agricultural College, Guangxi University, Nanning, 530005, China.
Wenfeng ZhaoState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Agricultural College, Guangxi University, Nanning, 530005, China.
Jianxin WeiState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Agricultural College, Guangxi University, Nanning, 530005, China.
Yibo XuState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Agricultural College, Guangxi University, Nanning, 530005, China.
Shixu ZhouState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Agricultural College, Guangxi University, Nanning, 530005, China.
Zihan WeiState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Agricultural College, Guangxi University, Nanning, 530005, China.
Zejun ShenState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Agricultural College, Guangxi University, Nanning, 530005, China.
Minghang WuState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Agricultural College, Guangxi University, Nanning, 530005, China.
Lianguang ShangAgricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
Rongbai LiState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Agricultural College, Guangxi University, Nanning, 530005, China.
Yongfei WangAgricultural Mechanization Service Center, Bama Yao Autonomous County, Guangxi Zhuang Autonomous Region, Bama, 547500, China.
Baoxiang QinState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Agricultural College, Guangxi University, Nanning, 530005, China. bxqin@gxu.edu.cn.ORCID http://orcid.org/0000-0001-8574-0849

Funding

Centrally Guided Local Science and Technology Development Fund ZY23055028Guangxi Natural Science Foundation 2024GXNSFGA010003Innovation Project of Guangxi Graduate Education YCBZ2022016State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources SKLCUSA-b202306the National Natural Science Foundation of China 31971809the National Natural Science Foundation of China 32372023the Project of Bama County for Talents in Science and Technology No.20220009
6 · The paper itself

Abstract

The fatty acyl ACP thioesterases, catalyzing the final step of fatty acid synthesis in the plastid, regulate various critical processes in plants, including seed oil accumulation, seed development, plant growth, and drought tolerance. However, their roles in male fertility have seldom been demonstrated. In this study, the function of a newly FAT, Impaired Pollen Fertility 1 (IPF1) in male fertility was investigated. IPF1 expressed prominently in microspores and tapetum. IPF1 specifically located in the endoplasmic reticulum. IPF1 knock-out mutants produced by the CRISPR/Cas9 system displayed significant reduction in seed-setting rate compared to WT. The decreased seed-setting rate in the ipf1 mutants was found to be attributed to the defects of pollen viability, not the female gamete fertility. The aborted pollen in the ipf1 mutants showed impaired pollen wall formation and diminished lipid deposition. Consistently, the expression levels of six genes critical to pollen wall formation and lipid metabolism (GPAT3, OsC6, DPW2, OsPKS1, OsPKS2, and OsSTRL2) were significantly decreased in the ipf1 mutant. Taken together, these results demonstrate that IPF1 regulates rice pollen fertility through the modulation of lipid synthesis.

Indexed as

Endoplasmic ReticulumOryzaPlant InfertilityPlant ProteinsThiolester HydrolasesCRISPR-Cas SystemsGene Expression Regulation, PlantLipid MetabolismMutationPollenSeedsPlant ProteinsThiolester HydrolasesIPF1Lipid biosynthesisPollen wall developmentRiceSeed-setting rate

Identifiers

PMID40056293

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.