Evidence map›Paper›PMID 40055760›Full record

ArticleMicrobial cell factories2025

Rewiring Escherichia coli to transform formate into methyl groups.

Michael K F Mohr, Ari Satanowski, Steffen N Lindner, Tobias J Erb, Jennifer N Andexer

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial cell factories, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Michael K F MohrInstitute of Pharmaceutical Sciences, University of Freiburg, Albertstr. 25, 79104, Freiburg, Germany.
Ari SatanowskiMax Planck Institute for Terrestrial Microbiology, Karl-Von-Frisch-Straße 10, 35043, Marburg, Germany.
Steffen N LindnerDepartment of Biochemistry, Charité Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität, Charitéplatz 1, 10117, Berlin, Germany.
Tobias J ErbMax Planck Institute for Terrestrial Microbiology, Karl-Von-Frisch-Straße 10, 35043, Marburg, Germany.
Jennifer N AndexerInstitute of Pharmaceutical Sciences, University of Freiburg, Albertstr. 25, 79104, Freiburg, Germany. jennifer.andexer@pharmazie.uni-freiburg.de.

Funding

Deutsche Bundesstiftung Umwelt 20021/742Deutsche Forschungsgemeinschaft 527572100European Research Council 716966
6 · The paper itself

Abstract

backgroundBiotechnological applications are steadily growing and have become an important tool to reinvent the synthesis of chemicals and pharmaceuticals for lower dependence on fossil resources. In order to sustain this progression, new feedstocks for biotechnological hosts have to be explored. One-carbon (C

resultsWe first used a two-vector system to link formate assimilation and SAM-dependent methylation with three different MTs in E. coli BL21. By feeding isotopically labelled formate, methylated products with 51-81%

conclusionsWithin this study transformation of formate into methyl groups is demonstrated in E. coli. Our findings support that feeding formate can improve the availability of usable C

Indexed as

Escherichia coliFormatesMetabolic EngineeringMethylationMethylobacterium extorquensMethyltransferasesS-AdenosylmethionineTetrahydrofolatesFormatesformic acidMethyltransferasesS-AdenosylmethionineTetrahydrofolatesBiotransformationC1-building blockFormic acidMethylationMethyltransferaseSAM

Identifiers

PMID40055760
PMCPMC11887345

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.