Evidence map›Paper›PMID 40055307›Full record

ArticleNature communications2025

Structure and dynamics of GAD65 in complex with an autoimmune polyendocrine syndrome type 2-associated autoantibody.

Susanne H D Ständer, Cyril F Reboul, Sarah N Le, Daniel E Williams, Peter G Chandler, Mauricio G S Costa, David E Hoke, John D T Jimma, James Fodor, Gustavo Fenalti and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
  5. State-of-the-Art and Future Directions in Structural Proteomics.Molecular & cellular proteomics : MCP · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Susanne H D Ständer *Protein Analysis Group, Department of Pharmacy, University of Copenhagen, Copenhagen, Denmark.
Cyril F Reboul *Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.ORCID http://orcid.org/0000-0002-6258-2697
Sarah N Le *Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.
Daniel E WilliamsDepartment of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.ORCID http://orcid.org/0000-0003-0577-2301
Peter G ChandlerDepartment of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.ORCID http://orcid.org/0000-0002-8978-3392
Mauricio G S CostaPrograma de Computação Científica, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil.ORCID http://orcid.org/0000-0001-5443-286X
David E HokeDepartment of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.
John D T JimmaProtein Analysis Group, Department of Pharmacy, University of Copenhagen, Copenhagen, Denmark.
James FodorDepartment of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.ORCID http://orcid.org/0000-0002-7159-1413
Gustavo FenaltiDepartment of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.
Stuart I ManneringImmunology and Diabetes Unit, St. Vincent's Institute of Medical Research, Fitzroy, Melbourne, VIC, Australia.ORCID http://orcid.org/0000-0003-3497-3559
Benjamin T PorebskiDepartment of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.
Peter SchofieldGarvan Institute of Medical Research, UNSW Sydney, Darlinghurst, NSW, Australia.
Daniel ChristGarvan Institute of Medical Research, UNSW Sydney, Darlinghurst, NSW, Australia.ORCID http://orcid.org/0000-0002-7313-3977
Malcolm BuckleLBPA, ENS de Paris-Saclay, UMR 8113 CNRS, Université Paris-Saclay 4, Gif-sur-Yvette, France.
Sheena McGowanDepartment of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.ORCID http://orcid.org/0000-0001-6863-1106
Dominika ElmlundDepartment of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.
Kasper D RandProtein Analysis Group, Department of Pharmacy, University of Copenhagen, Copenhagen, Denmark. kasper.rand@sund.ku.dk.ORCID http://orcid.org/0000-0002-6337-5489
Ashley M BuckleDepartment of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia. ashley.buckle@monash.edu.ORCID http://orcid.org/0000-0003-2943-9044

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The enzyme glutamate decarboxylase (GAD) produces the neurotransmitter GABA, using pyridoxal-5'-phosphate (PLP). GAD exists as two isoforms, GAD65 and GAD67. Only GAD65 acts as a major autoantigen, frequently implicated in type 1 diabetes and other autoimmune diseases. Here we characterize the structure and dynamics of GAD65 and its interaction with the autoimmune polyendocrine syndrome type 2-associated autoantibody b96.11. Using hydrogen-deuterium exchange mass spectrometry (HDX), X-ray crystallography, cryo-electron microscopy, and computational approaches, we examine the conformational dynamics of apo- and holoGAD65 and the GAD65-autoantibody complex. HDX reveals local dynamics accompanying autoinactivation, with the catalytic loop promoting collective motions at the CTD-PLP domain interface. In the GAD65-b96.11 complex, heavy chain CDRs dominate the interaction, with a long CDRH3 bridging the GAD65 dimer via electrostatic interactions with the

Indexed as

AutoantibodiesGlutamate DecarboxylasePolyendocrinopathies, AutoimmuneAutoantigensCryoelectron MicroscopyCrystallography, X-RayHumansHydrogen Deuterium Exchange-Mass SpectrometryProtein ConformationAutoantibodiesAutoantigensGlutamate Decarboxylaseglutamate decarboxylase 2

Identifiers

PMID40055307
PMCPMC11889217

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.