Evidence map›Paper›PMID 40055277›Full record

ArticleWorld journal of microbiology & biotechnology2025

Molecular profiling and bioinformatics approaches of biofilm formation in ionizing radiation-resistant Bacillus subtilis, isolated from geothermal spring in Ramsar, the North of Iran.

Dhuha Saeed Ali, Reza Vazifehmand, Maysoon Abulwahid Malik, Yaya Rukayadi, Son Radu, Mirsasan Mirpour, Mahmud Ab Rashid Nor-Khaizura

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Article in World journal of microbiology & biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Dhuha Saeed AliHalal Products Research Institute, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
Reza VazifehmandDepartment of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
Maysoon Abulwahid MalikDepartment of Plant Protection, Faculty of Agriculture, Razi University, Kermanshah, Iran.
Yaya RukayadiDepartment of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
Son RaduDepartment of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
Mirsasan MirpourDepartment of Microbiology, Faculty of Science, Islamic Azad University, Lahijan Branch, Lahijan, Gilan, Iran.
Mahmud Ab Rashid Nor-KhaizuraDepartment of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. norkhaizura@upm.edu.my.

Funding

Universiti Putra Malaysia Insentif Putra Siswazah, GP-IPS/2016/9488300
6 · The paper itself

Abstract

Biofilm formation and its molecular signaling in bacteria resistant to ionizing radiation is not fully understood. This study aimed to investigate the genetic variations and gene expression of biofilm in an ionizing radiation-resistant Bacillus subtilis in Ramsar. Direct sequencing and quantitative PCR were applied to determine nucleotide variations and gene expression profiles of tapA-sipW-tasA, sinR, sinI, ccpA, epsA-O, spoOB, spoOA, slrA, slrR, ymcA and abrB genes. RNAsnp-RNAfold and Phyre2 and the Swiss Model webserver were used to analyze the structural mRNA and protein respectively. At the molecular level, the tapA-sipW-tasA operon was significantly overexpressed and the expression of ccpA and slrR was significantly downregulated. The thermodynamic and ensemble diversity ratio of the tapA (G>C) gene showed the largest changes in RNA secondary structure. In addition, the largest protein pocket belonged to tapA (148.6 A

Indexed as

Bacillus subtilisBiofilmsHot SpringsBacterial ProteinsComputational BiologyGene Expression ProfilingGene Expression Regulation, BacterialGenetic VariationIranOperonRadiation, IonizingBacterial ProteinsBacillus subtilisBiofilm formationIonizing-radiation-resistantIranMolecular profiling

Identifiers

PMID40055277

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.