Evidence map›Paper›PMID 40054855›Full record

ArticleJournal of the American Chemical Society2025

Photochemistry of Receptor-Bound Flavin Resolved in Living Human Cells by Infrared Spectroscopy.

Lukas Goett-Zink, Lennard Karsten, Charlotte Mann, Hendrik Horstmeier, Jonas Spang, Kristian M Müller, Tilman Kottke

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the American Chemical Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Lukas Goett-ZinkBiophysical Chemistry and Diagnostics, Faculty of Chemistry, Bielefeld University, Bielefeld 33615, Germany.ORCID 0000-0002-0076-5308
Lennard KarstenCellular and Molecular Biotechnology, Faculty of Technology, Bielefeld University, Bielefeld 33615, Germany.
Charlotte MannCellular and Molecular Biotechnology, Faculty of Technology, Bielefeld University, Bielefeld 33615, Germany.
Hendrik HorstmeierBiophysical Chemistry and Diagnostics, Faculty of Chemistry, Bielefeld University, Bielefeld 33615, Germany.
Jonas SpangBiophysical Chemistry and Diagnostics, Faculty of Chemistry, Bielefeld University, Bielefeld 33615, Germany.
Kristian M MüllerCellular and Molecular Biotechnology, Faculty of Technology, Bielefeld University, Bielefeld 33615, Germany.
Tilman KottkeBiophysical Chemistry and Diagnostics, Faculty of Chemistry, Bielefeld University, Bielefeld 33615, Germany.ORCID 0000-0001-8080-9579

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In-cell experiments on proteins have revealed that the cellular environment can exert a considerable influence on protein mechanism and structure. Here, we introduce in-cell infrared difference spectroscopy (ICIRD) as a method to study soluble receptors in living human embryonic kidney cells by applying the attenuated total reflection approach. We demonstrate on the sensory domains of plant cryptochrome and aureochrome1a, a light, oxygen, or voltage (LOV) protein, that experiments can be performed using stable and transient transfection. Cells were cultivated and transfected on an internal reflection element directly inside the spectrometer, while their viability and growth were monitored

Indexed as

CryptochromesFlavinsHEK293 CellsHumansPhotochemical ProcessesSpectrophotometry, InfraredCryptochromesFlavins

Identifiers

PMID40054855
PMCPMC11926855

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.