Evidence map›Paper›PMID 40054472›Full record

ArticleStem cell reports2025

Robust generation of photoreceptor-dominant retinal organoids from porcine induced pluripotent stem cells.

Kimberly L Edwards, Bethany M Moore, Tyler-Serie Ganser, Praveen Joseph Susaimanickam, Kai Sovell, Yolana Martin, Lindsey D Jager, Ashley M Willes, Tyra H Moyer, Lydia Bowar and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Replicable generation and stable maintenance of rhesus macaque iPSCs forFrontiers in cell and developmental biology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Kimberly L EdwardsMcPherson Eye Research Institute, University of Wisconsin-Madison, Madison, WI, USA; Waisman Center, University of Wisconsin-Madison, Madison, WI, USA.
Bethany M MooreMorgridge Institute for Research, University of Wisconsin-Madison, Madison, WI, USA.
Tyler-Serie GanserWaisman Center, University of Wisconsin-Madison, Madison, WI, USA.
Praveen Joseph SusaimanickamMcPherson Eye Research Institute, University of Wisconsin-Madison, Madison, WI, USA; Waisman Center, University of Wisconsin-Madison, Madison, WI, USA.
Kai SovellWaisman Center, University of Wisconsin-Madison, Madison, WI, USA.
Yolana MartinWaisman Center, University of Wisconsin-Madison, Madison, WI, USA.
Lindsey D JagerWaisman Center, University of Wisconsin-Madison, Madison, WI, USA.
Ashley M WillesWaisman Center, University of Wisconsin-Madison, Madison, WI, USA.
Tyra H MoyerWaisman Center, University of Wisconsin-Madison, Madison, WI, USA.
Lydia BowarWaisman Center, University of Wisconsin-Madison, Madison, WI, USA.
M Joseph PhillipsMcPherson Eye Research Institute, University of Wisconsin-Madison, Madison, WI, USA; Waisman Center, University of Wisconsin-Madison, Madison, WI, USA.
Ron StewartMorgridge Institute for Research, University of Wisconsin-Madison, Madison, WI, USA.
Li-Fang ChuVeterinary Medicine, University of Calgary, Calgary, AB, Canada; Reproductive Biology and Regenerative Medicine Research Group, University of Calgary, Calgary, AB, Canada; Alberta Children's Hospital Research Institute, University of Calgary, Calgary, AB, Canada; Biochemistry and Molecular Biology Graduate Program, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada. Electronic address: lifangjack.chu@ucalgary.ca.
David M GammMcPherson Eye Research Institute, University of Wisconsin-Madison, Madison, WI, USA; Waisman Center, University of Wisconsin-Madison, Madison, WI, USA; Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, WI, USA. Electronic address: dgamm@wisc.edu.

Funding

UW COMPREHENSIVE CANCER CENTER SUPPORTP30CA014520 · NCI · UNIVERSITY OF WISCONSIN-MADISON · PI Justine Yang Bruce · 1985 to 2026
$142.6M
Mouse Neurodevelopmental Behavior CoreU54HD090255 · NICHD · BOSTON CHILDREN'S HOSPITAL · PI SAHIN, MUSTAFA · 2016 to 2020
$5.2M
Vision Research Training ProgramT32EY027721 · NEI · UNIVERSITY OF WISCONSIN-MADISON · PI ROBERT W NICKELLS · 2018 to 2026
$1.5M
NCI NIH HHS P30 CA014520NEI NIH HHS T32 EY027721NICHD NIH HHS U54 HD090255
6 · The paper itself

Abstract

Outer retinal degenerative diseases (RDDs) and injuries leading to photoreceptor (PR) loss are prevailing causes of blindness worldwide. While significant progress has been made in the manufacture of human pluripotent stem cell (hPSC)-derived PRs, robust production of pluripotent stem cell (PSC)-PRs from swine, a popular preclinical large animal model, would provide an avenue to collect conspecific functional and safety data to complement human xenograft studies. Toward this goal, we describe the highly efficient generation of PR-dominant porcine induced PSC (piPSC)-derived retinal organoids (ROs) using modifications of our established hPSC-RO differentiation protocol. Porcine iPSC-ROs were characterized using immunocytochemistry (ICC) and single-cell RNA sequencing (scRNA-seq), which revealed the presence and maturation of major neural retina cell types, including PRs and retinal ganglion cells, which possess molecular signatures akin to those found in hPSC-ROs. In late piPSC-ROs, a highly organized outer neuroepithelium was observed with rods and cones possessing outer segments and axon terminals expressing pre-synaptic markers adjacent to dendritic terminals of bipolar cells. The existence of piPSC lines and protocols that support reproducible, scalable production of female and male ROs will facilitate transplant studies in porcine models of retinal injury and RDDs unconfounded by immunological and evolutionary incompatibilities inherent to human xenografts.

Indexed as

Induced Pluripotent Stem CellsOrganoidsRetinaAnimalsCell DifferentiationHumansSwineallograftcell transplantationiPSC-derived retinal cellsneural retinaphotoreceptorsporcine iPSCsretinal differentiationretinal organoidsscRNA-seq

Identifiers

PMID40054472
PMCPMC12069893

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.