ArticleCell reports. Medicine2025
siRNA-AGO2 complex inhibits bacterial gene translation: A promising therapeutic strategy for superbug infection.
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Article in Cell reports. Medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7citing papers in PubMed
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Who cites it
7 citing papers in PubMed.
- Nucleic Acid-Based Antimicrobial Strategies Against Drug-Resistant Staphylococcus aureus: A Review of Mechanisms of Action and Delivery Approaches.Infectious diseases and therapy · 2026Review
- Antisense peptide nucleic acids targeting ribosomal protein genes: A novel strategy against carbapenem-resistantMolecular therapy. Nucleic acids · 2026Article
- `Successful treatment of VRE-infected mice with vancomycin through restoration of susceptibility using vanA antisense RNA.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- A TCER-1-siRNA regulatory axis suppresses antibacterial innate immunity in C. elegans.PLoS pathogens · 2026Article
- Review
- Biofluid-derived exosomes as next-generation biotherapeutic platforms to combat multidrug-resistant bacterial infections.Regenerative biomaterials · 2026Review
- Trans-Kingdom RNA Dialogues: miRNA and milRNA Networks as Biotechnological Tools for Sustainable Crop Defense and Pathogen Control.Plants (Basel, Switzerland) · 2025Review
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Authors and funding
22 authors.
Chen WangNanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Research Unit of Extracellular RNA, Chinese Academy of Medical Sciences, State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute of Life Sciences (NAILS), NJU Institute of Artificial Intelligence Biomedicine and Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210023, China; School of Pharmacy, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
Wangjian ShengState Key Laboratory of Coordination Chemistry, Jiangsu Key Laboratory of Advanced Organic Materials, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu 210023, China.
Yu ZhouNanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Research Unit of Extracellular RNA, Chinese Academy of Medical Sciences, State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute of Life Sciences (NAILS), NJU Institute of Artificial Intelligence Biomedicine and Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210023, China.
Xudong HangNHC Key Laboratory of Tropical Disease Control, School of Tropical Medicine, Hainan Medical University, Haikou, Hainan 571199, China.
Jiayi ZhaoNanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Research Unit of Extracellular RNA, Chinese Academy of Medical Sciences, State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute of Life Sciences (NAILS), NJU Institute of Artificial Intelligence Biomedicine and Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210023, China.
Yuanyuan GuNanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Research Unit of Extracellular RNA, Chinese Academy of Medical Sciences, State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute of Life Sciences (NAILS), NJU Institute of Artificial Intelligence Biomedicine and Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210023, China.
Xiangfeng MengNanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Research Unit of Extracellular RNA, Chinese Academy of Medical Sciences, State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute of Life Sciences (NAILS), NJU Institute of Artificial Intelligence Biomedicine and Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210023, China.
Yuefan BaiDepartment of Pathogen Biology, Jiangsu Key Laboratory of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu 211166, China.
Weili LiNanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Research Unit of Extracellular RNA, Chinese Academy of Medical Sciences, State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute of Life Sciences (NAILS), NJU Institute of Artificial Intelligence Biomedicine and Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210023, China.
Yujing ZhangNanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Research Unit of Extracellular RNA, Chinese Academy of Medical Sciences, State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute of Life Sciences (NAILS), NJU Institute of Artificial Intelligence Biomedicine and Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210023, China.
Linlin ZhangDepartment of Microbiology, Wu Lien-Teh Institute, Heilongjiang Provincial Key Laboratory of Infection and Immunity, Key Laboratory of Pathogen Biology, Harbin Medical University, Harbin, Heilongjiang 150081, China.
Jing YuNanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Research Unit of Extracellular RNA, Chinese Academy of Medical Sciences, State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute of Life Sciences (NAILS), NJU Institute of Artificial Intelligence Biomedicine and Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210023, China.
Zhen ZhouNanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Research Unit of Extracellular RNA, Chinese Academy of Medical Sciences, State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute of Life Sciences (NAILS), NJU Institute of Artificial Intelligence Biomedicine and Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210023, China.
Xiaona LiNanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Research Unit of Extracellular RNA, Chinese Academy of Medical Sciences, State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute of Life Sciences (NAILS), NJU Institute of Artificial Intelligence Biomedicine and Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210023, China.
Haorui SunNanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Research Unit of Extracellular RNA, Chinese Academy of Medical Sciences, State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute of Life Sciences (NAILS), NJU Institute of Artificial Intelligence Biomedicine and Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210023, China.
Yanhong XueNational Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Tao XuNational Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Ke ZenNanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Research Unit of Extracellular RNA, Chinese Academy of Medical Sciences, State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute of Life Sciences (NAILS), NJU Institute of Artificial Intelligence Biomedicine and Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210023, China.
Hong LingDepartment of Microbiology, Wu Lien-Teh Institute, Heilongjiang Provincial Key Laboratory of Infection and Immunity, Key Laboratory of Pathogen Biology, Harbin Medical University, Harbin, Heilongjiang 150081, China. Electronic address: lingh@ems.hrbmu.edu.cn.
Chen-Yu ZhangNanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Research Unit of Extracellular RNA, Chinese Academy of Medical Sciences, State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute of Life Sciences (NAILS), NJU Institute of Artificial Intelligence Biomedicine and Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210023, China. Electronic address: cyzhang@nju.edu.cn.
Hongkai BiNHC Key Laboratory of Tropical Disease Control, School of Tropical Medicine, Hainan Medical University, Haikou, Hainan 571199, China; Department of Pathogen Biology, Jiangsu Key Laboratory of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu 211166, China. Electronic address: hkbi@njmu.edu.cn.
Huan WangState Key Laboratory of Coordination Chemistry, Jiangsu Key Laboratory of Advanced Organic Materials, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu 210023, China. Electronic address: wanghuan@nju.edu.cn.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Silencing resistance genes of pathogenic bacteria by RNA interference (RNAi) is a potential strategy to fight antibiotic-resistant bacterial infections. Currently, RNAi cannot be achieved in bacteria due to the lack of RNA-induced silencing complex machinery and the difficulty of small interfering RNA (siRNA) delivery. Here, we show that exosomal siRNAs can be efficiently delivered into bacterial cells and can silence target genes primarily through translational repression without mRNA degradation. The exosomal Argonaute 2 (AGO2) protein forms a complex with siRNAs, which is essential for bacterial gene silencing. Both in vitro and in vivo-generated exosome-packaged siRNAs resensitize methicillin-resistant Staphylococcus aureus (MRSA) to methicillin treatment by silencing the mecA gene, which is the primary beta-lactam resistance determinant of MRSA. This approach significantly enhances the therapeutic effect in a mouse model of MRSA infection. In summary, our study provides a method for siRNA delivery to bacteria that may facilitate the treatment of antibiotic-resistant bacterial infection.
Indexed as
Argonaute ProteinsMethicillin-Resistant Staphylococcus aureusProtein BiosynthesisRNA, Small InterferingStaphylococcal InfectionsAnimalsAnti-Bacterial AgentsBacterial ProteinsDisease Models, AnimalExosomesHumansMicePenicillin-Binding ProteinsRNA InterferenceAGO2 protein, humanAnti-Bacterial AgentsArgonaute ProteinsBacterial ProteinsmecA protein, Staphylococcus aureusPenicillin-Binding ProteinsRNA, Small InterferingAGOantibiotic resistanceexosomegene silencingin vivo self-assembled siRNAMRSARNA deliverysmall RNAtranslational repression
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