Evidence map›Paper›PMID 40053704›Full record

ArticleThe journal of physical chemistry. B2025

Growth of Clusters toward Liquid-Liquid Phase Separation of Monoclonal Antibodies as Characterized by Small-Angle X-ray Scattering and Molecular Dynamics Simulation.

William D Kimball, Alfredo Lanzaro, Christian Hurd, Neel Jhaveri, Jintian Huang, Joshua Lewandowski, Ken K Qian, Mahlet A Woldeyes, Ranajoy Majumdar, Marta A Witek and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The journal of physical chemistry. B, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

William D KimballMcKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas 78712, United States.ORCID 0009-0004-4133-2068
Alfredo LanzaroMcKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas 78712, United States.
Christian HurdMcKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas 78712, United States.
Neel JhaveriMcKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas 78712, United States.
Jintian HuangMcKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas 78712, United States.
Joshua LewandowskiMcKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas 78712, United States.
Ken K QianEli Lilly and Company, Indianapolis, Indiana 46225, United States.
Mahlet A WoldeyesEli Lilly and Company, Indianapolis, Indiana 46225, United States.
Ranajoy MajumdarEli Lilly and Company, Indianapolis, Indiana 46225, United States.
Marta A WitekEli Lilly and Company, Indianapolis, Indiana 46225, United States.
Jiangyan FengEli Lilly and Company, Indianapolis, Indiana 46225, United States.
Richard E GillilanCenter for High Energy X-ray Sciences at CHESS, Cornell University, Ithaca, New York 14853, United States.
Qingqiu HuangCenter for High Energy X-ray Sciences at CHESS, Cornell University, Ithaca, New York 14853, United States.
Alexander E MarrasWalker Department of Mechanical Engineering, The University of Texas at Austin, Austin, Texas 78712, United States.ORCID 0000-0001-8972-9532
Thomas M TruskettMcKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas 78712, United States.ORCID 0000-0002-6607-6468
Keith P JohnstonMcKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas 78712, United States.ORCID 0000-0002-0915-1337

Funding

Training and OutreachP30GM124166 · NIGMS · CORNELL UNIVERSITY · PI RICHARD A. CERIONE · 2019 to 2026
$28.3M
NIGMS NIH HHS P30 GM124166
6 · The paper itself

Abstract

In concentrated protein solutions, short-range attractions (SRAs) contribute to liquid-liquid phase separation (LLPS) as a function of temperature and salinity, particularly when the charge and thus long-range repulsions are low near the isoelectric point pI. Herein, we study how SRA and solution morphology vary with the approach to LLPS from increased SRA for two monoclonal antibodies (mAbs) as salt concentration is reduced near the pI. These properties are quantified using small-angle X-ray scattering (SAXS) interpreted via coarse-grained (CG) molecular dynamics (MD) simulations and compared with less descriptive properties from static and dynamic light scattering. Experimental structure factors are fit with a library of MD simulations for a CG 12-bead mAb model to determine the SRA strength (

Indexed as

Antibodies, MonoclonalMolecular Dynamics SimulationHydrogen-Ion ConcentrationPhase SeparationScattering, Small AngleX-Ray DiffractionAntibodies, Monoclonal

Identifiers

PMID40053704
PMCPMC12186533

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.