Evidence map›Paper›PMID 40053450›Full record

ArticleSTAR protocols2025

Protocol for characterizing airway epithelial ciliary beating and mucociliary transport using image processing and particle imaging velocimetry.

Quoc Vo, Kambez H Benam

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Quoc VoDivision of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Kambez H BenamDivision of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA 15213, USA; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15219, USA; Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA 15213, USA. Electronic address: kambez.benam@pitt.edu.

Funding

Emulating Immune Dysregulation by Trisomy 21 in a Multi-Organ-on-a-Chip SystemR01HL159494 · NHLBI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI HAJIPOURAN BENAM, KAMBEZ · 2021 to 2025
$3.6M
A Microphysiological Mimicry of Human Lung-Bone Marrow Organ-Organ Crosstalk On-a-ChipU01EB029085 · NIBIB · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI HAJIPOURAN BENAM, KAMBEZ · 2019 to 2023
$2.0M
NHLBI NIH HHS R01 HL159494NIBIB NIH HHS U01 EB029085
6 · The paper itself

Abstract

Lung epithelial cilia play a crucial role in respiratory health by driving mucociliary transport (MCT) and clearing inhaled pathogens and materials. Here, we present a protocol for measuring both ciliary beat frequency (CBF) and MCT in mucociliary human small airway epithelial cells. We describe steps for configuring experimental setups, collecting CBF and MCT data, analyzing datasets, and performing post-acquisition analysis. This protocol is adaptable to mucociliary cells from other lung regions and to mouse tracheal epithelial cells. For complete details on the use and execution of this protocol, please refer to Thomas et al.

Indexed as

CiliaEpithelial CellsImage Processing, Computer-AssistedMucociliary ClearanceRespiratory MucosaRheologyAnimalsHumansMiceTracheabiotechnology and bioengineeringmicroscopytissue engineering

Identifiers

PMID40053450
PMCPMC11930158

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.