ArticleJournal of animal science2025
Technical note: optimizing and validating an RP-HPLC method to determine lactoferrin in porcine colostrum and milk.
Article in Journal of animal science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
This Technical Note provides a detailed description of a sample preparation procedure, along with the validation of a reversed-phase high-performance liquid chromatography (RP-HPLC) method for quantitatively determining lactoferrin (LF) in porcine colostrum and milk. The analysis of native milk samples is a challenging process due to the complex composition of the sample. Raw milk is an emulsion and colloid of fat globules in a water-based liquid containing dissolved carbohydrates and protein aggregates with minerals. This paper aims to optimize a method for preparing porcine colostrum and milk samples, which involves a new combination of homogenization, centrifugation, dilution, and filtration techniques for the subsequent analysis of LF via RP-HPLC. A validation process was implemented to ensure the reliability and repeatability of this analytical approach. The results showed that the quantification of LF content in porcine colostrum (1,684.58 ± 466.68 µg/mL; n = 10) and milk (849.75 ± 85.82 µg/mL; n = 10) is feasible with successful validation. In addition, the improved sensitivity due to the novel combination of these preparation steps is shown by low limits of detection and quantification of 0.21 µg/mL each. The advantage of this optimized methodology is that the sample preparation can be carried out relatively simply and using standard laboratory equipment, thus enabling the accurate determination of LF in porcine colostrum and milk samples.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.