Evidence map›Paper›PMID 40050695›Full record

ArticleCommunications biology2025

Genetically-encoded markers for confocal visualization of single dense core vesicles.

Junwei Yu, Yunpeng Zhang, Kelsey Clements, Nannan Chen, Leslie C Griffith

Abstract read
In one paragraph

Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Soma CaCurrent biology : CB · 2026
    Article
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Junwei Yu *Department of Biology, Volen National Center for Complex Systems, Brandeis University, Waltham, MA, USA.
Yunpeng Zhang *Department of Biology, Volen National Center for Complex Systems, Brandeis University, Waltham, MA, USA.
Kelsey ClementsDepartment of Biology, Volen National Center for Complex Systems, Brandeis University, Waltham, MA, USA.
Nannan ChenSchool of Life Science and Technology, Key Laboratory of Developmental Genes and Human Disease, Southeast University, Nanjing, China. nannanchen27@seu.edu.cn.ORCID http://orcid.org/0009-0003-9915-552X
Leslie C GriffithDepartment of Biology, Volen National Center for Complex Systems, Brandeis University, Waltham, MA, USA. griffith@brandeis.edu.ORCID http://orcid.org/0000-0003-3164-9876

Funding

Resource Component: Acquisition, maintenance and distribution of Drosophila stocksP40OD018537 · OD · TRUSTEES OF INDIANA UNIVERSITY · PI Cale Whitworth · 2014 to 2026
$13.5M
Neurotransmitter Plasticity and Regulation of BehaviorR01NS122970 · NINDS · BRANDEIS UNIVERSITY · PI Leslie C Griffith · 2022 to 2026
$2.0M
Genetic tools for visualization of co-transmission in identified neuronsR21NS096414 · NINDS · BRANDEIS UNIVERSITY · PI GRIFFITH, LESLIE C · 2016 to 2017
$447k
Identifying the Mechanisms and Localization of Activity-Dependent CaMKII SynthesisF31NS134189 · NINDS · BRANDEIS UNIVERSITY · PI CLEMENTS, KELSEY · 2023 to 2025
$116k
NIH HHS P40 OD018537NINDS NIH HHS F31 NS134189NINDS NIH HHS R01 NS122970NINDS NIH HHS R21 NS096414U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS) R21NS096414
6 · The paper itself

Abstract

Neuronal dense core vesicles (DCVs) store and release a diverse array of neuromodulators, trophic factors, and bioamines. The analysis of single DCVs has largely been possible only using electron microscopy, which makes understanding cargo segregation and DCV heterogeneity difficult. To address these limitations, we develop genetically encoded markers for DCVs that can be used in combination with standard immunohistochemistry and expansion microscopy to enable single-vesicle resolution with confocal microscopy in Drosophila.

Indexed as

Drosophila melanogasterNeuronsSecretory VesiclesAnimalsAnimals, Genetically ModifiedBiomarkersDrosophilaDrosophila ProteinsImmunohistochemistryMicroscopy, ConfocalBiomarkersDrosophila Proteins

Identifiers

PMID40050695
PMCPMC11885565

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.