Evidence map›Paper›PMID 40050626›Full record

ArticleScientific reports2025

Preliminary survey of biofilm forming, antibiotic resistant Escherichia coli in fishes from land based aquaculture systems and open water bodies in Bangladesh.

Md Liton Rana, Md Ashek Ullah, M Nazmul Hoque, Jayedul Hassan, Mahbubul Pratik Siddique, Md Tanvir Rahman

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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Md Liton RanaDepartment of Microbiology and Hygiene, Faculty of Veterinary Sciences, Agricultural University, 2202, Mymensingh, Bangladesh.
Md Ashek UllahDepartment of Microbiology and Hygiene, Faculty of Veterinary Sciences, Agricultural University, 2202, Mymensingh, Bangladesh.
M Nazmul HoqueMolecular Biology and Bioinformatics Laboratory, Department of Gynecology, Obstetrics and Reproductive Health, Faculty of Veterinary Medicine and Animal Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, 1706, Gazipur, Bangladesh.
Jayedul HassanDepartment of Microbiology and Hygiene, Faculty of Veterinary Sciences, Agricultural University, 2202, Mymensingh, Bangladesh.
Mahbubul Pratik SiddiqueDepartment of Microbiology and Hygiene, Faculty of Veterinary Sciences, Agricultural University, 2202, Mymensingh, Bangladesh.
Md Tanvir RahmanDepartment of Microbiology and Hygiene, Faculty of Veterinary Sciences, Agricultural University, 2202, Mymensingh, Bangladesh. tanvirahman@bau.edu.bd.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The emergence and spread of multidrug-resistant pathogens, such as Escherichia coli, present major global public health challenges. This study aimed to investigate the prevalence, antibiotic resistance patterns, biofilm production, and the presence of antibiotic resistance genes (ARGs) and biofilm-forming genes in E. coli isolated from fish in open-body water (wild) sources and land-based aquaculture (cultured) systems in Mymensingh, Bangladesh. We collected 130 fish (Koi: Anabas testudineus and Shing: Heteropneustes fossilis) among which 70 were from wild sources and 60 from cultured systems. We screened 116 probable E. coli isolates through selective culture, Gram-staining, and biochemical tests. Using malB gene-specific PCR, we confirmed 87 isolates (67.0%) as E. coli. Cultured fish had a higher prevalence (70.0%) compared to wild fish (64.0%). Biofilm formation was detected in 20.0% E. coli by Congo red agar tests. However, crystal violet assays revealed that 70.0% of E. coli from cultured fish produced biofilm, compared to 20.0% from wild fish, with 7.0% of cultured fish isolates showing strong biofilm production. Antibiotic resistance profiling showed that 100.0% E. coli isolates were resistant to ampicillin and ceftazidime, beta-lactamase-producing antibiotics. Resistance patterns varied by source, with nearly 97.0% of E. coli from cultured fish being multidrug-resistant (MDR), compared to 60.0% in wild fish. E. coli from cultured fish were identified as potential reservoirs of ARGs such as blaTEM (83.0%), blaSHV (81.0%), blaCTX (78.57%), and the biofilm forming gene fimC (100.0%). Significant associations were observed for blaTEM (p = 0.033), blaSHV (p = 0.038), and fimC (p = 0.005). These findings highlight the need for monitoring β-lactamase-resistant and biofilm-forming E. coli in both wild and cultured fish in Bangladesh due to their potential threat to public health and animal populations.

Indexed as

BiofilmsDrug Resistance, BacterialEscherichia coliEscherichia coli InfectionsFishesAnimalsAnti-Bacterial AgentsAquacultureBangladeshDrug Resistance, Multiple, BacterialFish DiseasesMicrobial Sensitivity TestsWater MicrobiologyAnti-Bacterial AgentsAntibiotic resistanceBangladeshBiofilm productionE.coliPublic healthWild and cultured fish

Identifiers

PMID40050626
PMCPMC11885838

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LicenceCC BY-NC-ND
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.