Evidence map›Paper›PMID 40050447›Full record

ArticleArchives of virology2025

Molecular characterization of a polymycovirus in Leptosphaeria biglobosa.

Nesma M Samir, Jacob Locke-Gotel, Syun-Ichi Urayama, Adel A El-Morsi, Ghada A El-Sherbeny, Yongju Huang, Bruce D L Fitt, Hiromitsu Moriyama, Robert H A Coutts, Ioly Kotta-Loizou

Abstract read
In one paragraph

Article in Archives of virology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Diverse, novel mycoviruses coinfecting the phytopathogenic fungusFrontiers in cellular and infection microbiology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Nesma M SamirLaboratory of Molecular and Cellular Biology, Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, 3-5-8, Saiwaicho, Fuchu, Tokyo, 183-8509, Japan.
Jacob Locke-GotelDepartment of Clinical, Pharmaceutical and Biological Science, School of Life and Medical Sciences, University of Hertfordshire, Hatfield, AL10 9AB, UK.
Syun-Ichi UrayamaLaboratory of Fungal Interaction and Molecular Biology (donated by IFO), Department of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, Japan.
Adel A El-MorsiDepartment of Botany, Faculty of science, Mansoura University, Mansoura, 35516, Egypt.
Ghada A El-SherbenyDepartment of Botany, Faculty of science, Mansoura University, Mansoura, 35516, Egypt.
Yongju HuangDepartment of Clinical, Pharmaceutical and Biological Science, School of Life and Medical Sciences, University of Hertfordshire, Hatfield, AL10 9AB, UK.
Bruce D L FittDepartment of Clinical, Pharmaceutical and Biological Science, School of Life and Medical Sciences, University of Hertfordshire, Hatfield, AL10 9AB, UK.
Hiromitsu MoriyamaLaboratory of Molecular and Cellular Biology, Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, 3-5-8, Saiwaicho, Fuchu, Tokyo, 183-8509, Japan.
Robert H A CouttsDepartment of Clinical, Pharmaceutical and Biological Science, School of Life and Medical Sciences, University of Hertfordshire, Hatfield, AL10 9AB, UK.
Ioly Kotta-LoizouDepartment of Clinical, Pharmaceutical and Biological Science, School of Life and Medical Sciences, University of Hertfordshire, Hatfield, AL10 9AB, UK. i.kotta-loizou2@herts.ac.uk.ORCID http://orcid.org/0000-0003-3277-6359

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Leptosphaeria biglobosa is a phytopathogenic ascomycete of Brassica napus that causes phoma stem canker/blackleg. A new double-stranded RNA (dsRNA) mycovirus from this fungus has been fully characterized. The virus genome has five dsRNA segments, ranging in length from 1,180 bp to 2,402 bp. Each dsRNA has a single open reading frame flanked by 5' and 3' untranslated regions. The proteins encoded by dsRNAs 1 and 3, an RNA-dependent RNA polymerase (RdRP) and a methyltransferase, respectively, have significant similarity to those of Plasmopara viticola lesion associated polymycovirus 1. The proline-alanine-serine-rich protein encoded by dsRNA 5 is similar to that of Erysiphe necator associated polymycovirus 1. The proteins encoded by dsRNAs 2 and 4 have significant similarity to those of a mycovirus identified in Alternaria sp. FA0703. Phylogenetic analysis based on RdRP sequences showed that this virus clusters with members of the family Polymycoviridae. Based on these observations, this virus, which we have named "Leptosphaeria biglobosa polymycovirus 1", should be classified as a member of the family Polymycoviridae. This is the first report of a polymycovirus in L. biglobosa.

Indexed as

AscomycotaDouble Stranded RNA VirusesFungal VirusesBrassica napusGenome, ViralOpen Reading FramesPhylogenyPlant DiseasesRNA-Dependent RNA PolymeraseRNA, Double-StrandedRNA, ViralViral ProteinsRNA-Dependent RNA PolymeraseRNA, Double-StrandedRNA, ViralViral Proteins

Identifiers

PMID40050447
PMCPMC11885375

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.