Evidence map›Paper›PMID 40050327›Full record

ArticleScientific reports2025

3D virtual histology of rodent and primate cochleae with multi-scale phase-contrast X-ray tomography.

Jannis J Schaeper, Christoph A Kampshoff, Bettina J Wolf, Lennart Roos, Susann Michanski, Torben Ruhwedel, Marina Eckermann, Alexander Meyer, Marcus Jeschke, Carolin Wichmann and 2 more

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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Jannis J SchaeperInstitute for X-Ray Physics, University of Göttingen, 37077, Göttingen, Germany.
Christoph A KampshoffDepartment of Otolaryngology, University Medical Center Göttingen, 37075, Göttingen, Germany.
Bettina J WolfInstitute for Auditory Neuroscience and Inner Ear Lab, University Medical Center Göttingen, 37075, Göttingen, Germany.
Lennart RoosDepartment of Otolaryngology, University Medical Center Göttingen, 37075, Göttingen, Germany.
Susann MichanskiInstitute for Auditory Neuroscience and Inner Ear Lab, University Medical Center Göttingen, 37075, Göttingen, Germany.
Torben RuhwedelMax Planck Institute for Multidisciplinary Sciences, 37075, Göttingen, Germany.
Marina EckermannBeamline ID16A, European Synchrotron Radiation Facility, 38000, Grenoble, France.
Alexander MeyerDepartment of Otolaryngology, University Medical Center Göttingen, 37075, Göttingen, Germany.
Marcus JeschkeInstitute for Auditory Neuroscience and Inner Ear Lab, University Medical Center Göttingen, 37075, Göttingen, Germany.
Carolin WichmannInstitute for Auditory Neuroscience and Inner Ear Lab, University Medical Center Göttingen, 37075, Göttingen, Germany.
Tobias MoserDepartment of Otolaryngology, University Medical Center Göttingen, 37075, Göttingen, Germany.
Tim SaldittInstitute for X-Ray Physics, University of Göttingen, 37077, Göttingen, Germany. tsaldit@gwdg.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Multi-scale X-ray phase contrast tomography (XPCT) enables three-dimensional (3D), non-destructive imaging of intact small animal cochlea and apical cochlear turns. Here we report on post-mortem imaging of excised non-human primate and rodent cochleae at different [Formula: see text]-CT and nano-CT synchrotron instruments. We explore different sample embeddings, stainings and imaging regimes. Under optimized conditions of sample preparation, instrumentation, imaging protocol, and phase retrieval, high image quality and detail level can be achieved in 3D reconstructions. The showcased instrumentation and imaging protocols along with the reconstucted volumes can serve as benchmarks and reference for multi-scale microanatomy and 3D histology. The provided benchmarks and imaging protocols of this work cover a wide range of scales and are intended as augmented imaging tools for auditory research.

Indexed as

CochleaImaging, Three-DimensionalTomography, X-Ray ComputedAnimalsMicePrimatesRatsCochlea imagingMicro-CTPhase contrastSpiral ganglion neuronsSynchrotron radiationX-ray tomography

Identifiers

PMID40050327
PMCPMC11885485

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.