Evidence map›Paper›PMID 40050299›Full record

ArticleScientific reports2025

Establishment & characterization of a non-adherent insect cell line for cultivated meat.

Sophia M Letcher, Olivia P Calkins, Halla J Clausi, Aidan McCreary, Barry A Trimmer, David L Kaplan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Sophia M LetcherDepartment of Biomedical Engineering, Tufts University, Medford, MA, 02155, USA.
Olivia P CalkinsDepartment of Chemical Engineering, Tufts University, Medford, MA, 02155, USA.
Halla J ClausiDepartment of Biomedical Engineering, Tufts University, Medford, MA, 02155, USA.
Aidan McCrearyDepartment of Chemistry, Tufts University, Medford, MA, 02155, USA.
Barry A TrimmerDepartment of Biomedical Engineering, Tufts University, Medford, MA, 02155, USA.
David L KaplanDepartment of Biomedical Engineering, Tufts University, Medford, MA, 02155, USA. David.kaplan@tufts.edu.

Funding

New Harvest noneUS Department of Agriculture 2021-05678
6 · The paper itself

Abstract

This study presents a blueprint for developing, scaling, and analyzing novel insect cell lines for food. The large-scale production of cultivated meat requires the development and analysis of cell lines that are simple to grow and easy to scale. Insect cells may be a favorable cell source due to their robust growth properties, adaptability to different culture conditions, and resiliency in culture. Cells were isolated from Tobacco hornworm (Manduca sexta) embryos and subsequently adapted to single-cell suspension culture in animal-free growth media. Cells were able to reach relatively high cell densities of over 20 million cells per mL in shake flasks. Cell growth data is presented in various culture vessels and spent media analysis was performed to better understand cell metabolic processes. Finally, a preliminary nutritional profile consisting of proximate, amino acid, mineral, and fatty acid analysis is reported.

Indexed as

Cell Culture TechniquesManducaMeatAnimalsCell LineCulture MediaFatty AcidsIn Vitro MeatCulture MediaFatty AcidsCellular agricultureCultivated meatCultured meatInsect cell cultureNutritionSuspension culture

Identifiers

PMID40050299
PMCPMC11885424

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.