ArticleNature communications2025
Energy filtering enables macromolecular MicroED data at sub-atomic resolution.
Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed.
- Characterization of energy filtering slit widths for MicroED data collection.Journal of structural biology: X · 2026Article
- Atomic resolution cryo-EM at 200 keV.IUCrJ · 2026Article
- Towards time-resolved MicroED grid preparation using mix-and-inject gas dynamic virtual nozzles.IUCrJ · 2026Article
- Ultra-rapid cryo-EM data acquisition method enabled by continuous recording based beam image shift.Nature communications · 2026Article
- Membrane protein structure and dynamics probed by MicroED.Biochemical Society transactions · 2026Review
- Advances of 3D microcrystals electron diffraction for transmembrane protein structure determination.Biophysical journal · 2026Review
- Single-cell structural biology with intracellular electron crystallography.Nature communications · 2026Article
- Direct from the seed: an atomic resolution protein structure by ab initio MicroED.Nature communications · 2026Article
- Standards for MicroED.Acta crystallographica. Section C, Structural chemistry · 2025Article
- Ligand Screening and Discovery Using Cocktail Soaking and Automated Microcrystal Electron Diffraction.ChemMedChem · 2025Article
- Recovering high-resolution information using energy filtering in MicroED.Structural dynamics (Melville, N.Y.) · 2025Article
Corrections and comments
- Erratum issued
- Update of
Authors and funding
4 authors.
Funding
Abstract
High-resolution information is important for accurate structure modeling but is challenging to attain in macromolecular crystallography due to the rapid fading of diffracted intensities at increasing resolution. While direct electron detection essentially eliminates the read-out noise during MicroED data collection, other sources of noise remain and limit the measurement of faint high-resolution reflections. Inelastic scattering significantly contributes to noise, raising background levels and broadening diffraction peaks. We demonstrate a substantial improvement in signal-to-noise ratio by using energy filtering to remove inelastically scattered electrons. This strategy results in sub-atomic resolution MicroED data from proteinase K crystals, enabling the visualization of detailed structural features. Interestingly, reducing the noise further reveals diffuse scattering that may hold additional structural information. Our findings suggest that combining energy filtering and direct detection provides more accurate measurements at higher resolution, facilitating precise model refinement and improved insights into protein structure and function.
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