Engineered phenylalanine hydroxylase coupled with an effective cofactor synthesis and regeneration system for high-yield production of 5-hydroxytryptophan.

Yulin Ai, Yusong Huang, Hongru Zhao, Bingmei Su, Juan Lin

Abstract read

In one paragraph

Article in Bioresources and bioprocessing, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yulin AiCollege of Biological Science and Engineering, Fuzhou University, Fuzhou, 350108, China.

Yusong HuangCollege of Chemical Engineering, Fuzhou University, Fuzhou, 350108, China.

Hongru ZhaoCollege of Chemical Engineering, Fuzhou University, Fuzhou, 350108, China.

Bingmei SuCollege of Biological Science and Engineering, Fuzhou University, Fuzhou, 350108, China. subingmei@fzu.edu.cn.

Juan LinCollege of Biological Science and Engineering, Fuzhou University, Fuzhou, 350108, China. ljuan@fzu.edu.cn.ORCID http://orcid.org/0000-0003-4202-285X

Funding

National Natural Science Foundation of China 22208058

6 · The paper itself

Abstract

5-Hydroxytryptophan (5-HTP) is widely used as a natural remedy for sleep disorders. In terms of biosafety, bio-derived 5-HTP is preferred over chemically synthesized 5-HTP. However, the low titer of 5-HTP in the reported microbiological methods (< 10 g/L) limits the industrialization of 5-HTP biosynthesis. In the present study, a Trp-accumulating E. coli strain TRP1 was constructed by blocking the degradation path (ΔtnaA), branching paths (ΔpheA, ΔtyrA) and repression system (ΔtrpR, ΔtrpL). Next, the hydroxylation module employing a phenylalanine hydroxylase mutant XcPAH

Indexed as

5-HydroxytryptophanCofactor engineeringGenome editingPhenylalanine hydroxylaseProtein engineering

Identifiers

PMID40047997

DOI10.1186/s40643-025-00846-z

PMCPMC11885754

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.