Evidence map›Paper›PMID 40047967›Full record

ArticleVeterinary research communications2025

Validation and comparison of EvaGreen- and TaqMan-based real-time qRT-PCR for diagnosis of the black queen cell virus in the honey bees.

Mustafa Emin Oz, Oguzhan Avci

Abstract readValidation StudyComparative Study
PubMed Publisher
In one paragraph

Article in Veterinary research communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Mustafa Emin OzMolecular Microbiology Laboratory, Konya Veterinary Control Institute, Konya, 42090, Türkiye. mustafaemin.oz@tarimorman.gov.tr.ORCID http://orcid.org/0000-0001-7919-8643
Oguzhan AvciMolecular Microbiology Laboratory, Konya Veterinary Control Institute, Konya, 42090, Türkiye.ORCID http://orcid.org/0000-0001-9299-4695

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The black queen cell virus (BQCV) is a common agent that causes covert infection in hives and has a global distribution. In this study, TaqMan probe and EvaGreen (EG) dye-based real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays based on the amplification of coding senquences for the BQCV structural protein (SP) and the non-structural protein (NSP) were developed and validated for the detection of BQCV in adult bee and larvae/pupae. Additionally, the performances of commonly used EG and TaqMan chemicals for real-time qRT-PCR analyses were compared. The operating range for BQCV-SP TaqMan and EG real-time qRT-PCRs ranged 2.61-7.61 log

Indexed as

DicistroviridaeReal-Time Polymerase Chain ReactionAnimalsBeesLarvaPupaSensitivity and SpecificityBQCVDiagnosisEvaGreen dyeHoney beeReal-time qRT-PCRTaqMan probe

Identifiers

PMID40047967

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.