Evidence map›Paper›PMID 40047936›Full record

ArticleLung2025

FLRT3 Overexpression Attenuates Ischemia-Reperfusion Induced Vascular Hyperpermeability and Lung Injury Through RND3.

Yongmei Cao, Shiyang Sheng, Yong Zhong, Jiawei Shang, Cui Jin, Qin Tan, Feng Ping, Weifeng Huang, Yongchao Liu, Yingchuan Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Lung, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yongmei Cao *Department of Critical Care Medicine, Shanghai Tenth People'S Hospital, Tongji University School of Medicine, No. 301, Middle Yanchang Road, Jingan District, Shanghai, 200072, China.
Shiyang Sheng *Department of Critical Care Medicine, Shanghai Tenth People'S Hospital, Tongji University School of Medicine, No. 301, Middle Yanchang Road, Jingan District, Shanghai, 200072, China.
Yong Zhong *Clinical Nuclear Medicine Center, Imaging Clinical Medical Center, Institute of Nuclear Medicine, Department of Nuclear Medicine, Shanghai Tenth People'S Hospital, Tongji University School of Medicine, Shanghai, 200072, China.
Jiawei ShangDepartment of Critical Care Medicine, Shanghai Sixth People'S Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, No. 605, Yishan Road, Xuhui District, Shanghai, 200233, China.
Cui JinDepartment of Critical Care Medicine, Shanghai Sixth People'S Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, No. 605, Yishan Road, Xuhui District, Shanghai, 200233, China.
Qin TanDepartment of Critical Care Medicine, Shanghai Sixth People'S Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, No. 605, Yishan Road, Xuhui District, Shanghai, 200233, China.
Feng PingDepartment of Critical Care Medicine, Shanghai Sixth People'S Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, No. 605, Yishan Road, Xuhui District, Shanghai, 200233, China.
Weifeng HuangDepartment of Critical Care Medicine, Shanghai Sixth People'S Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, No. 605, Yishan Road, Xuhui District, Shanghai, 200233, China. breeze-huang@hotmail.com.
Yongchao LiuDepartment of Critical Care Medicine, Shanghai Tenth People'S Hospital, Tongji University School of Medicine, No. 301, Middle Yanchang Road, Jingan District, Shanghai, 200072, China. liuyongchao@tongji.edu.cn.
Yingchuan LiDepartment of Critical Care Medicine, Shanghai Tenth People'S Hospital, Tongji University School of Medicine, No. 301, Middle Yanchang Road, Jingan District, Shanghai, 200072, China. yingchuan_li@tongji.edu.cn.

Funding

Clinical technology innovation project of Shanghai Shenkang Hospital Development Center SHDC22021203Exploration of the Application of Quality Management Tools in Intensive Care Medicine Education under the Context of High-Quality Development 2024-B-12National Natural Science Foundation of China 82272245Natural Science Foundation of Shanghai 21Y11902800Shanghai Chongming District Sustainable Development and Technological Innovation Action Plan Project CKY2024-36Technical standardization management and promotion project of Shanghai Shenkang Hospital Development Center SHDC22023211
6 · The paper itself

Abstract

purposePulmonary ischemia/reperfusion injury (IRI) causes endothelial barrier dysfunction and increased vascular permeability. Fibronectin leucine-rich transmembrane protein-3 (FLRT3) is known to regulate endothelial cell function, but its role in pulmonary IRI remains unexplored.

methodsWe established both a mouse lung I/R model and a hypoxia/reoxygenation (H/R) cell culture model using human pulmonary microvascular endothelial cells (HPMECs). The effects of FLRT3 manipulation were assessed through lentiviral-mediated overexpression and knockdown approaches. Lung injury was evaluated by histological analysis, immunohistochemistry, and lung injury scoring. Endothelial barrier function was assessed using transmission electron microscopy, Evans blue extravasation, and endothelial permeability assays.

resultsFLRT3 expression was predominantly localized in pulmonary endothelial cells and was downregulated following I/R injury. Lentiviral vectors overexpressing FLRT3 (LV-FLRT3, 1 × 10

conclusionsOur findings reveal FLRT3, a critical regulator of endothelial barrier function during IRI through the RND3-RhoA pathway, is a potential therapeutic target for pulmonary IRI.

Indexed as

Capillary PermeabilityLung InjuryReperfusion Injuryrho GTP-Binding ProteinsAnimalsAutophagyCells, CulturedDisease Models, AnimalEndothelial CellsHumansLungMaleMiceMice, Inbred C57BLrhoA GTP-Binding ProteinSignal TransductionrhoA GTP-Binding Proteinrho GTP-Binding ProteinsRND3 protein, humanCytoskeletal rearrangementFLRT3Lung ischemia/reperfusionRND3Vascular permeability

Identifiers

PMID40047936
PMCPMC11885377

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.