Evidence map›Paper›PMID 40044788›Full record

ArticleScientific reports2025

Molecular delineation and haplotype analysis of domain membrane protein (DMP) gene influencing in-vivo haploid induction in maize.

Nisrita Gain, Rashmi Chhabra, Vignesh Muthusamy, Suman Dutta, Konsam Sarika, Elangbam L Devi, Jayanthi Madhavan, Rajkumar U Zunjare, Aruna Kumar, Devendra K Yadava and 1 more

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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Nisrita GainICAR-Indian Agricultural Research Institute, New Delhi, India.
Rashmi ChhabraICAR-Indian Agricultural Research Institute, New Delhi, India.
Vignesh MuthusamyICAR-Indian Agricultural Research Institute, New Delhi, India.
Suman DuttaICAR-Indian Agricultural Research Institute, New Delhi, India.
Konsam SarikaICAR Research Complex for NEH Region, Manipur Centre, Lamphelpat, India.
Elangbam L DeviICAR Research Complex for NEH Region, Sikkim Centre, Gangtok, India.
Jayanthi MadhavanICAR-Indian Agricultural Research Institute, New Delhi, India.
Rajkumar U ZunjareICAR-Indian Agricultural Research Institute, New Delhi, India.
Aruna KumarAmity Institute of Biotechnology, AMITY University, Noida, India.
Devendra K YadavaIndian Council of Agricultural Research (ICAR), New Delhi, India.
Firoz HossainICAR-Indian Agricultural Research Institute, New Delhi, India. fh_gpb@yahoo.com.

Funding

Department of Biotechnology, Govt. of India BT/PR25134/NER/95/1035/2017Indian Council of Agricultural Research 24-142G
6 · The paper itself

Abstract

Domain membrane protein (DMP) gene is one of the key genes regulating in vivo haploid production in maize. Here, full-length sequence (931 bp) of DMP gene was sequenced in five mutant and five wild-type maize inbreds to study allelic variation. Two SNPs viz., T to C (SNP1) and G to A (SNP2) that distinguished wild-type and mutant- allele of DMP gene, converted methionine to threonine and alanine to threonine at positions 44 and 87, respectively. Two breeder-friendly PCR-based markers (DMP_SNP_TC and DMP_SNP_GA) specific to SNP1 and SNP2 were developed. These markers identified four haplotypes, viz., Hap-DMP-TG, Hap-DMP-CG, Hap-DMP-TA, and Hap-DMP-CA among a set of 48 diverse maize inbreds. Both SNP1 and SNP2 were present in DUF679_motif-2 and DUF679_motif-1, respectively and were conserved among maize and its paralogues, hence resulting in altered protein. Analysis with 11 paralogues of maize showed high similarity with DMP gene. Comparison with 11 paralogues revealed that maize DUF679 protein of DMP gene was more similar to dmp_Zm_Zm00001eb110420 paralogue. Gene structure analysis showed that most of the DMP genes have single exon. The breeder-friendly markers developed in the present study can be utilized in marker-assisted introgression of DMP gene to develop new haploid inducer lines in maize. This is the first report on identification of novel SNP differentiating the wild-type and mutant allele of DMP gene, besides comprehensive molecular characterization of the DMP gene in maize and its paralogues.

Indexed as

HaploidyHaplotypesMembrane ProteinsPlant ProteinsZea maysAllelesPolymorphism, Single NucleotideMembrane ProteinsPlant ProteinsDHDMPHaploidsMotifsParalogues

Identifiers

PMID40044788
PMCPMC11882960

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