Evidence map›Paper›PMID 40044748›Full record

ArticleScientific reports2025

Progressive T cell exhaustion and predominance of aging tissue associated macrophages with advancing disease stage in penile squamous cell carcinoma.

Hiroko Miyagi, Xiaoqing Yu, Taylor Peak, Jasreman Dhillon, Casey Le, Xuefeng Wang, Sean Yoder, Doug Marchion, Xin Lu, Curtis Pettaway and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Hiroko Miyagi *Genitourinary Oncology Department, H. Lee Moffitt Cancer Center, 12902 Magnolia Dr, Tampa, FL, 33612, USA. Hiroko.miyagi@moffitt.org.
Xiaoqing Yu *Department of Biostatistics and Bioinformatics, H. Lee Moffitt Cancer Center, Tampa, FL, 33612, USA.
Taylor PeakDepartment of Urology, University of Tennessee College of Medicine, Chatanooga, USA.
Jasreman DhillonPathology Department, H. Lee Moffitt Cancer Center, Tampa, FL, 33612, USA.
Casey LeGenitourinary Oncology Department, H. Lee Moffitt Cancer Center, 12902 Magnolia Dr, Tampa, FL, 33612, USA.
Xuefeng WangDepartment of Biostatistics and Bioinformatics, H. Lee Moffitt Cancer Center, Tampa, FL, 33612, USA.
Sean YoderMolecular Genomics Core, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, USA.
Doug MarchionTissue Core Shared Resource, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, USA.
Xin LuDepartment of Biological Sciences, University of Notre Dame, Norte Dame, IN, 46556, USA.
Curtis PettawayDepartment of Urology, University of Texas MD Anderson Cancer Center, Houston, TX, 77030, USA.
Carlos Moran SeguraPathology Department, H. Lee Moffitt Cancer Center, Tampa, FL, 33612, USA.
Chaomei ZhangMolecular Genomics Core, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, USA.
Gabriel Roman SouzaGenitourinary Oncology Department, H. Lee Moffitt Cancer Center, 12902 Magnolia Dr, Tampa, FL, 33612, USA.
Alice YuGenitourinary Oncology Department, H. Lee Moffitt Cancer Center, 12902 Magnolia Dr, Tampa, FL, 33612, USA.
Logan ZempGenitourinary Oncology Department, H. Lee Moffitt Cancer Center, 12902 Magnolia Dr, Tampa, FL, 33612, USA.
Philippe Spiess *Genitourinary Oncology Department, H. Lee Moffitt Cancer Center, 12902 Magnolia Dr, Tampa, FL, 33612, USA.
Jad Chahoud *Genitourinary Oncology Department, H. Lee Moffitt Cancer Center, 12902 Magnolia Dr, Tampa, FL, 33612, USA. Jad.chahoud@moffitt.org.

Funding

TRANSLATIONAL RESEARCHP30CA076292 · NCI · UNIVERSITY OF SOUTH FLORIDA · PI Christine O'Connell · 1998 to 2026
$93.5M
NCI NIH HHS P30 CA076292
6 · The paper itself

Abstract

Penile squamous cell carcinoma (PSCC) is a rare malignancy with limited understanding of the tumor immune microenvironment (TIME). The interplay between PSCC and the immune system across disease progression and HPV infection status remains poorly characterized. This study aims to assess the TIME changes from localized to advanced disease and between HPV-positive versus negative tumors to identify potential immune evasion mechanisms in advanced PSCC. scRNA-seq was performed on ten PSCC tissue samples from penile, lymph node and distant metastatic sites with four matched penile and lymph node samples to understand the cellular heterogeneity within PSCC tumors. Analysis of immune cell populations and transcriptional hallmarks were performed stratified by localized (pT1-3, N0) versus advanced (N1-3, M0 or any N, M1) disease states and HPV infection status. We observed significant differences in immune cell infiltration between localized and advanced PSCC disease states and by HPV status. Advanced disease states demonstrated an exhausted immune phenotype, characterized by terminally exhausted CD8

Indexed as

Carcinoma, Squamous CellCD8-Positive T-LymphocytesMacrophagesPenile NeoplasmsAgedDisease ProgressionHumansLymphocytes, Tumor-InfiltratingMaleMiddle AgedNeoplasm StagingPapillomavirus InfectionsT-Cell ExhaustionTumor MicroenvironmentCancer progressionHuman papillomavirusImmune microenvironmentPenile squamous cell carcinomaSingle-cell RNA sequencing

Identifiers

PMID40044748
PMCPMC11882776

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.