Evidence map›Paper›PMID 40044221›Full record

ArticleGenome research2025

Homozygous editing of multiple genes for accelerated generation of xenotransplantation pigs.

Xiaoyue Duan, Chaolei Chen, Chang Du, Liang Guo, Jun Liu, Naipeng Hou, Pan Li, Xiaolan Qi, Fei Gao, Xuguang Du and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genome research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Efficient and Safe Knockout ofVeterinary sciences · 2026
    Article
  5. Review
  6. Review
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Xiaoyue Duan *Sanya Institute of China Agricultural University, Sanya, 572024, China.
Chaolei Chen *State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Chang Du *State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Liang Guo *Sanya Institute of China Agricultural University, Sanya, 572024, China.
Jun LiuState Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Naipeng HouSanya Institute of China Agricultural University, Sanya, 572024, China.
Pan LiSanya Institute of China Agricultural University, Sanya, 572024, China.
Xiaolan QiState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730000, China.
Fei GaoSanya Institute of China Agricultural University, Sanya, 572024, China.
Xuguang DuSanya Institute of China Agricultural University, Sanya, 572024, China; xuguangdu@cau.edu.cn fwsongjiangping@126.com swu@cau.edu.cn.
Jiangping SongSanya Institute of China Agricultural University, Sanya, 572024, China; xuguangdu@cau.edu.cn fwsongjiangping@126.com swu@cau.edu.cn.
Sen WuSanya Institute of China Agricultural University, Sanya, 572024, China; xuguangdu@cau.edu.cn fwsongjiangping@126.com swu@cau.edu.cn.ORCID 0000-0003-0764-6122

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Although CRISPR-Cas-based genome editing has made significant strides over the past decade, achieving simultaneous homozygous gene editing of multiple targets in primary cells remains a significant challenge. In this study, we optimized a coselection strategy to enhance homozygous gene editing rates in the genomes of primary porcine fetal fibroblasts (PFFs). The strategy utilizes the expression of a surrogate reporter (

Indexed as

Gene EditingTransplantation, HeterologousAnimalsAnimals, Genetically ModifiedCRISPR-Cas SystemsFibroblastsGalactosyltransferasesHomozygoteNuclear Transfer TechniquesSwineGalactosyltransferases

Identifiers

PMID40044221
PMCPMC12047534

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.